Anti-Sarcomeric Alpha Actinin antibody (ab72592)


  • Product nameAnti-Sarcomeric Alpha Actinin antibody
    See all Sarcomeric Alpha Actinin primary antibodies
  • Description
    Rabbit polyclonal to Sarcomeric Alpha Actinin
  • Tested applicationsSuitable for: IHC-Fr, WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from the N-terminal of human Sarcomeric Alpha Actinin.

  • Positive control
    • Human skeletal muscle tissue. Extracts from HeLa cells, HepG2 cells and HUVEC cells. HeLa cells.



Our Abpromise guarantee covers the use of ab72592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 22427522
WB 1/500 - 1/1000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.


  • FunctionF-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
  • Tissue specificityExpressed in both skeletal and cardiac muscle.
  • Involvement in diseaseDefects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similaritiesBelongs to the alpha-actinin family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
    Contains 4 spectrin repeats.
  • Cellular localizationCytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin binding protein antibody
    • Actinin alpha 2 antibody
    • ACTN 2 antibody
    • ACTN2 antibody
    • ACTN2_HUMAN antibody
    • Alpha actinin 2 antibody
    • Alpha actinin skeletal muscle antibody
    • Alpha actinin skeletal muscle isoform 2 antibody
    • Alpha-actinin skeletal muscle isoform 2 antibody
    • Alpha-actinin-2 antibody
    • CMD1AA antibody
    • F actin cross linking protein antibody
    • F-actin cross-linking protein antibody
    see all

Anti-Sarcomeric Alpha Actinin antibody images

  • All lanes : Anti-Sarcomeric Alpha Actinin antibody (ab72592) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells
    Lane 2 : Extracts from HepG2 cells
    Lane 3 : Extracts from HUVEC cells
    Lane 4 : Extracts from HUVEC cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size : 103 kDa
    Observed band size : 103 kDa
    Additional bands at : 80 kDa,85 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using ab72592, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
  • Immunofluorescent analysis of HeLa cells using ab72592, at 1/500 dilution, in the presence (right panel) or absence (left panel) of the immunising peptide.

References for Anti-Sarcomeric Alpha Actinin antibody (ab72592)

This product has been referenced in:
  • Verhaart IE  et al. The Dynamics of Compound, Transcript, and Protein Effects After Treatment With 2OMePS Antisense Oligonucleotides in mdx Mice. Mol Ther Nucleic Acids 3:e148 (2014). WB . Read more (PubMed: 24549299) »
  • Giudice J  et al. Alternative splicing regulates vesicular trafficking genes in cardiomyocytes during postnatal heart development. Nat Commun 5:3603 (2014). WB ; Mouse . Read more (PubMed: 24752171) »

See all 9 Publications for this product

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I have conducted a search for you and found several antibodies against Sarcomeric Alpha Actinin. One of them is ab9465 which has been tested in ICC/IF and it recognizes cardiac muscle i...

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Application Western blot
Sample Human Cell lysate - whole cell (normal and tumor colon cells)
Loading amount 20 µg
Specification normal and tumor colon cells
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Submitted Sep 12 2011