Anti-Sarcomeric Alpha Actinin antibody (ab72592)

Overview

  • Product name
    Anti-Sarcomeric Alpha Actinin antibody
    See all Sarcomeric Alpha Actinin primary antibodies
  • Description
    Rabbit polyclonal to Sarcomeric Alpha Actinin
  • Tested applications
    Suitable for: IHC-Fr, WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide derived from the N-terminal of human Sarcomeric Alpha Actinin.

  • Positive control
    • Human skeletal muscle tissue. Extracts from HeLa cells, HepG2 cells and HUVEC cells. HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab72592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 22427522
WB 1/500 - 1/1000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
ELISA 1/20000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • Function
    F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
  • Tissue specificity
    Expressed in both skeletal and cardiac muscle.
  • Involvement in disease
    Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similarities
    Belongs to the alpha-actinin family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
    Contains 4 spectrin repeats.
  • Cellular localization
    Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin binding protein antibody
    • Actinin alpha 2 antibody
    • ACTN 2 antibody
    • ACTN2 antibody
    • ACTN2_HUMAN antibody
    • Alpha actinin 2 antibody
    • Alpha actinin skeletal muscle antibody
    • Alpha actinin skeletal muscle isoform 2 antibody
    • Alpha-actinin skeletal muscle isoform 2 antibody
    • Alpha-actinin-2 antibody
    • CMD1AA antibody
    • F actin cross linking protein antibody
    • F-actin cross-linking protein antibody
    see all

Images

  • All lanes : Anti-Sarcomeric Alpha Actinin antibody (ab72592) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells
    Lane 2 : Extracts from HepG2 cells
    Lane 3 : Extracts from HUVEC cells
    Lane 4 : Extracts from HUVEC cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 103 kDa
    Observed band size : 103 kDa
    Additional bands at : 80 kDa,85 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using ab72592, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
  • Immunofluorescent analysis of HeLa cells using ab72592, at 1/500 dilution, in the presence (right panel) or absence (left panel) of the immunising peptide.

References

This product has been referenced in:
  • Giudice J  et al. Neonatal cardiac dysfunction and transcriptome changes caused by the absence of Celf1. Sci Rep 6:35550 (2016). WB ; Mouse . Read more (PubMed: 27759042) »
  • Quijada P  et al. Nuclear Calcium/Calmodulin-dependent Protein Kinase II Signaling Enhances Cardiac Progenitor Cell Survival and Cardiac Lineage Commitment. J Biol Chem 290:25411-26 (2015). WB ; Mouse . Read more (PubMed: 26324717) »

See all 16 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry and your interest in our products.
I have conducted a search for you and found several antibodies against Sarcomeric Alpha Actinin. One of them is ab9465 which has been tested in ICC/IF and it recognizes cardiac muscle i...

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Application
Western blot
Sample
Human Cell lysate - whole cell (normal and tumor colon cells)
Loading amount
20 µg
Specification
normal and tumor colon cells
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Sep 12 2011

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