Anti-Sarcomeric Alpha Actinin antibody (ab72592)
Key features and details
- Rabbit polyclonal to Sarcomeric Alpha Actinin
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Sarcomeric Alpha Actinin antibody
See all Sarcomeric Alpha Actinin primary antibodies -
Description
Rabbit polyclonal to Sarcomeric Alpha Actinin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide derived from the N-terminal of human Sarcomeric Alpha Actinin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72592 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/1000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/500 - 1/1000.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa). |
IHC-P
1/50 - 1/100. |
ICC/IF
1/500 - 1/1000. |
Target
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Function
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. -
Tissue specificity
Expressed in both skeletal and cardiac muscle. -
Involvement in disease
Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
Sequence similarities
Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
Cellular localization
Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle. - Information by UniProt
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Database links
- Entrez Gene: 88 Human
- Omim: 102573 Human
- SwissProt: P35609 Human
- Unigene: 498178 Human
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Alternative names
- Actin binding protein antibody
- Actinin alpha 2 antibody
- ACTN 2 antibody
see all
Images
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All lanes : Anti-Sarcomeric Alpha Actinin antibody (ab72592) at 1/500 dilution
Lane 1 : Extracts from HeLa cells
Lane 2 : Extracts from HepG2 cells
Lane 3 : Extracts from HUVEC cells
Lane 4 : Extracts from HUVEC cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 103 kDa
Observed band size: 103 kDa
Additional bands at: 80 kDa, 85 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue using ab72592, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.
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Immunofluorescent analysis of HeLa cells using ab72592, at 1/500 dilution, in the presence (right panel) or absence (left panel) of the immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (28)
ab72592 has been referenced in 28 publications.
- Signorelli M et al. Peripheral blood transcriptome profiling enables monitoring disease progression in dystrophic mice and patients. EMBO Mol Med 13:e13328 (2021). PubMed: 33751844
- Casas-Fraile L et al. Frizzled related protein deficiency impairs muscle strength, gait and calpain 3 levels. Orphanet J Rare Dis 15:119 (2020). PubMed: 32448375
- van Putten M et al. Nonclinical Exon Skipping Studies with 2'-O-Methyl Phosphorothioate Antisense Oligonucleotides in mdx and mdx-utrn-/- Mice Inspired by Clinical Trial Results. Nucleic Acid Ther 29:92-103 (2019). PubMed: 30672725
- Blue RE et al. Modulation of alternative splicing of trafficking genes by genome editing reveals functional consequences in muscle biology. Int J Biochem Cell Biol 105:134-143 (2018). PubMed: 30316870
- Veltrop M et al. A dystrophic Duchenne mouse model for testing human antisense oligonucleotides. PLoS One 13:e0193289 (2018). PubMed: 29466448