Anti-Sarcomeric Alpha Actinin antibody (ab90776)

Overview

  • Product name
    Anti-Sarcomeric Alpha Actinin antibody
    See all Sarcomeric Alpha Actinin primary antibodies
  • Description
    Rabbit polyclonal to Sarcomeric Alpha Actinin
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 850 to the C-terminus of Human Sarcomeric Alpha Actinin.

    (Peptide available as ab100828.)

  • Positive control
    • This antibody gave a positive signal in the following human tissue lysates: Skeletal Muscle; Heart.

Properties

Applications

Our Abpromise guarantee covers the use of ab90776 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).

Target

  • Function
    F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
  • Tissue specificity
    Expressed in both skeletal and cardiac muscle.
  • Involvement in disease
    Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similarities
    Belongs to the alpha-actinin family.
    Contains 1 actin-binding domain.
    Contains 2 CH (calponin-homology) domains.
    Contains 2 EF-hand domains.
    Contains 4 spectrin repeats.
  • Cellular localization
    Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin binding protein antibody
    • Actinin alpha 2 antibody
    • ACTN 2 antibody
    • ACTN2 antibody
    • ACTN2_HUMAN antibody
    • Alpha actinin 2 antibody
    • Alpha actinin skeletal muscle antibody
    • Alpha actinin skeletal muscle isoform 2 antibody
    • Alpha-actinin skeletal muscle isoform 2 antibody
    • Alpha-actinin-2 antibody
    • CMD1AA antibody
    • F actin cross linking protein antibody
    • F-actin cross-linking protein antibody
    see all

Images

  • All lanes : Anti-Sarcomeric Alpha Actinin antibody (ab90776) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 104 kDa
    Observed band size : 104 kDa
    Additional bands at : 27 kDa,30 kDa,45 kDa,80 kDa,90 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • IHC image of Sarcomeric Alpha Actinin staining in human Heart FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90776, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

References

This product has been referenced in:
  • Huang Y  et al. Age-Dependent Oxidative DNA Damage Does Not Correlate with Reduced Proliferation of Cardiomyocytes in Humans. PLoS One 12:e0170351 (2017). Read more (PubMed: 28099512) »
  • Liang P  et al. Patient-Specific and Genome-Edited Induced Pluripotent Stem Cell-Derived Cardiomyocytes Elucidate Single-Cell Phenotype of Brugada Syndrome. J Am Coll Cardiol 68:2086-2096 (2016). ICC/IF ; Human . Read more (PubMed: 27810048) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Pulmonary artery smooth muscle cell)
Specification
Pulmonary artery smooth muscle cell
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Verified customer

Submitted Apr 14 2014

Thank you very much for contacting us today. I am sorry that you have been experiencing problems with ab90776. The quality of our products is extremely important to us so thank for getting in touch. I can provide a vial from a different lot...

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