Full length native protein (purified) corresponding to Rabbit Sarcomeric Alpha Actinin. Purified rabbit skeletal α-actinin.
Our Abpromise guarantee covers the use of ab9465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/25 - 1/50. ABC method. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 17383364|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 20019242|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue staining Sarcomeric Alpha Actinin with ab9465.
Immunocytochemistry/Immunofluorescence analysis of H9 hESC and CBiPSC6.2 cells labelling Sarcomeric Alpha Actinin (green) with ab9465 at a dilution of 1/200. An Alexa Fluor® 488-conjugated goat anti-mouse IgG was used as the secondary antibody (1/200).