Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

Overview

  • Product nameAnti-Sarcomeric Alpha Actinin antibody [EP2529Y]
    See all Sarcomeric Alpha Actinin primary antibodies
  • Description
    Rabbit monoclonal [EP2529Y] to Sarcomeric Alpha Actinin
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Sarcomeric Alpha Actinin aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control
    • WB: Human skeletal muscle and fetal heart, mouse, rat and human heart and rat brain tissue lysates IHC-P: Human and mouse cardiac muscle tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab68167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).
IP 1/20 - 1/40.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

  • Application notesIs unsuitable for Flow Cyt or ICC/IF.
  • Target

    • FunctionF-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
    • Tissue specificityExpressed in both skeletal and cardiac muscle.
    • Involvement in diseaseDefects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    • Sequence similaritiesBelongs to the alpha-actinin family.
      Contains 1 actin-binding domain.
      Contains 2 CH (calponin-homology) domains.
      Contains 2 EF-hand domains.
      Contains 4 spectrin repeats.
    • Cellular localizationCytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.
    • Information by UniProt
    • Database links
    • Alternative names
      • Actin binding protein antibody
      • Actinin alpha 2 antibody
      • ACTN 2 antibody
      • ACTN2 antibody
      • ACTN2_HUMAN antibody
      • Alpha actinin 2 antibody
      • Alpha actinin skeletal muscle antibody
      • Alpha actinin skeletal muscle isoform 2 antibody
      • Alpha-actinin skeletal muscle isoform 2 antibody
      • Alpha-actinin-2 antibody
      • CMD1AA antibody
      • F actin cross linking protein antibody
      • F-actin cross-linking protein antibody
      see all

    Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] images

    • All lanes : Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/10000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size : 103 kDa
      Observed band size : 103 kDa

      Blocking and dilution buffer: 5% NFDM /TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue labelling Sarcomeric Alpha Actinin with purified ab68167 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/20000 dilution (purified) + Mouse heart tissue lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size : 103 kDa
      Observed band size : 103 kDa

      Blocking and dilution buffer: 5% NFDM /TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue labelling Sarcomeric Alpha Actinin with purified ab68167 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/20000 dilution (purified) + Human fetal heart tissue lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size : 103 kDa
      Observed band size : 103 kDa

      Blocking and dilution buffer: 5% NFDM /TBST.

    • ab68167 (purified) at 1/20 immunoprecipitating Sarcomeric Alpha Actinin in HepG2 whole cell lysate.

      Lane 1 (input): HepG2 whole cell lysate (10µg)

      Lane 2 (+): ab68167 + HepG2 whole cell lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab68167 in HepG2 whole cell lysate.

      For western blotting, ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/10000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167) at 1/20000 dilution (unpurified)

      Lane 1 : Human skeletal muscle lysate
      Lane 2 : Rat heart lysate
      Lane 3 : Human heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 103 kDa
      Observed band size : 103 kDa
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling Sarcomeric Alpha Actin with unpurified ab68167 at a dilution of 1/100.

    References for Anti-Sarcomeric Alpha Actinin antibody [EP2529Y] (ab68167)

    This product has been referenced in:
    • Cowan DB  et al. Intracoronary Delivery of Mitochondria to the Ischemic Heart for Cardioprotection. PLoS One 11:e0160889 (2016). IHC-P ; Rabbit . Read more (PubMed: 27500955) »
    • Canseco DC  et al. Human ventricular unloading induces cardiomyocyte proliferation. J Am Coll Cardiol 65:892-900 (2015). IHC-Fr ; Mouse . Read more (PubMed: 25618530) »

    See all 6 Publications for this product

    Product Wall

    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (heart)
    Permeabilization Yes - 0.3% Triton X
    Specification heart
    Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 20%
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 11 2015

    Western blot

    Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 4 µg
    Gel Running Conditions Reduced Denaturing (12% gel)
    Sample Mouse Cell lysate - whole cell (mouse cardiac cells. Cardiomyocytes and non-cardio)
    Specification mouse cardiac cells. Cardiomyocytes and non-cardio
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 23 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"