Recombinant
RabMAb

Anti-Sarcomeric Alpha Actinin antibody [EP2530Y] (ab68168)

Overview

  • Product name
    Anti-Sarcomeric Alpha Actinin antibody [EP2530Y]
    See all Sarcomeric Alpha Actinin primary antibodies
  • Description
    Rabbit monoclonal [EP2530Y] to Sarcomeric Alpha Actinin
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to residues near the C terminus of human Sarcomeric Alpha Actinin

  • Positive control
    • Human skeletal muscle lysate and human muscle tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab68168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    IP: 1/40.
    WB: 1/20000 - 1/50000. Detects a band of approximately 103 kDa (predicted molecular weight: 103 kDa).

    Is unsuitable for Flow Cyt or ICC/IF.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
    • Tissue specificity
      Expressed in both skeletal and cardiac muscle.
    • Involvement in disease
      Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    • Sequence similarities
      Belongs to the alpha-actinin family.
      Contains 1 actin-binding domain.
      Contains 2 CH (calponin-homology) domains.
      Contains 2 EF-hand domains.
      Contains 4 spectrin repeats.
    • Cellular localization
      Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle.
    • Information by UniProt
    • Database links
    • Alternative names
      • Actin binding protein antibody
      • Actinin alpha 2 antibody
      • ACTN 2 antibody
      • ACTN2 antibody
      • ACTN2_HUMAN antibody
      • Alpha actinin 2 antibody
      • Alpha actinin skeletal muscle antibody
      • Alpha actinin skeletal muscle isoform 2 antibody
      • Alpha-actinin skeletal muscle isoform 2 antibody
      • Alpha-actinin-2 antibody
      • CMD1AA antibody
      • F actin cross linking protein antibody
      • F-actin cross-linking protein antibody
      see all

    Images

    • Anti-Sarcomeric Alpha Actinin antibody [EP2530Y] (ab68168) at 1/50000 dilution + human skeletal muscle lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 103 kDa
      Observed band size : 103 kDa
    • ab68168 at 1/100 dilution staining Sarcomeric Alpha Actinin in human muscles by Immunohistochemistry, Paraffin-embedded tissue.

    References

    ab68168 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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