• Product name
    Sarcosine Assay Kit
  • Sample type
    Cell culture supernatant, Milk, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type
  • Sensitivity
    > 1 µM
  • Range
    1 µM - 10000 µM
  • Assay time
    1h 00m
  • Product overview

    Abcam's Sarcosine Assay Kit provides an accurate, convenient measure of sarcosine in variety biological samples. In the assay, sarcosine is specifically oxidized to generate a product that converts a colorless probe to a product with intense red color (?max = 570 nm) and which is also highly fluorescent (Ex/Em = 538/587 nm). Sarcosine is therefore easily detected by either colorimetric or fluorometric methods with detection range 1-10000 µM.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Sarcosine, a natural amino acid, plays important roles as intermediate in the metabolism of choline, methionine, glycine, glutathione, creatine, purine and serine, etc. Detection of sarcosine level has wide applications in research and development.

  • Tested applications
    Suitable for: Functional Studiesmore details


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components Identifier 100 tests
    Sarcosine Assay Buffer WM 1 x 30ml
    Sarcosine Enzyme Mix (Lyophilized) Green 1 vial
    Sarcosine Probe (in DMSO, anhydrous) Red 1 vial
    Sarcosine Standard (10 µmol) (Lyophilized) Yellow 1 vial
  • Research areas
  • Relevance
    Sarcosine (N-methylglycine) is an intermediate and byproduct in the synthesis and degradation of glycine. Sarcosine is metabolized to glycine by sarcosine dehydrogenase, while glycine-N-methyl transferase generates sarcosine from glycine. Sarcosine is a natural amino acid found in muscles and other body tissues and plays important role as an intermediate in the metabolism of choline, methionine, glycine, glutathione, creatine, purine and serine.
  • Alternative names
    • N methylglycine


Our Abpromise guarantee covers the use of ab65338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies
  • Application notes
    FuncS: Use at an assay dependent dilution.
    Note: Urine samples do not work well with the assay due to sample interferences.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Images

    • Sarcosine Standard Curve performed using ab65338.



    This product has been referenced in:
    • Barfeld SJ  et al. c-Myc Antagonises the Transcriptional Activity of the Androgen Receptor in Prostate Cancer Affecting Key Gene Networks. EBioMedicine 18:83-93 (2017). Read more (PubMed: 28412251) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Thank you for your enquiry. We have contacted our source to request details of the composition of the assay buffer, enzyme mix and probe and I am sorry they have regrettably informed me that this is commercially sensitive information and they a...

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    Thank you for your enquiry. Please find below the answers to your questions. 1. Biospecimens like body fluids (plasma/ serum), tissues, and cell culture samples can be used. However, urine samples will give interference with the assay. For c...

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