Anti-SARM antibody (ab17812)
Key features and details
- Rabbit polyclonal to SARM
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SARM antibody
See all SARM primary antibodies -
Description
Rabbit polyclonal to SARM -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide (Human) (C terminal).
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Positive control
- Daudi cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17812 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (4) |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 80 kDa.
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ICC/IF |
Use a concentration of 2 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 80 kDa. |
ICC/IF
Use a concentration of 2 µg/ml. |
Target
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Function
Involved in innate immnune response. Acts as a negative regulator of TICAM1/TRIF-dependent Toll-like receptor signaling by inhibiting induction of TLR3- and TLR4-dependent genes. Specifically blocks TICAM1/TRIF-dependent transcription-factor activation and gene induction, without affecting the MYD88-dependent pathway or non-TLR signaling. Negative regulator of NF-kappa-B and IRF activation. -
Tissue specificity
Predominantly expressed in kidney and liver. Expressed at lower level in placenta. -
Sequence similarities
Contains 2 SAM (sterile alpha motif) domains.
Contains 1 TIR domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 23098 Human
- Entrez Gene: 237868 Mouse
- Omim: 607732 Human
- SwissProt: Q6SZW1 Human
- SwissProt: Q6PDS3 Mouse
- Unigene: 446689 Human
- Unigene: 210332 Mouse
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Alternative names
- FLJ36296 antibody
- KIAA0524 antibody
- MyD88-5 antibody
see all
Images
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Western Blot of Daudi cell lysate labeling SARM with Anti-SARM antibody (ab17812) at (1) 0.5 and (2) 1µg/ml.
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Lane 1 : Anti-SARM antibody (ab17812) at 0.5 µg/ml
Lane 2 : Anti-SARM antibody (ab17812) at 1 µg/ml
Lane 3 : Anti-SARM antibody (ab17812) at 2 µg/ml
All lanes : Daudi cell lysate
Predicted band size: 80 kDa
Observed band size: 80 kDa
Western blot analysis of SARM (79 kDa) in Daudi cell lysates with ab17812 at (A) 0.5, (B) 1, and (C) 2µ g/ml. -
ab17812 at 2µg/ml staining of SARM in Daudi cells by ICC/IF
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab17812 has been referenced in 3 publications.
- Luo K et al. MiR-149-5p regulates cisplatin chemosensitivity, cell growth, and metastasis of oral squamous cell carcinoma cells by targeting TGFß2. Int J Clin Exp Pathol 12:3728-3739 (2019). PubMed: 31933761
- Liu M et al. Overexpression of miR-422a inhibits cell proliferation and invasion, and enhances chemosensitivity in osteosarcoma cells. Oncol Rep 36:3371-3378 (2016). PubMed: 27779704
- de Vos AF et al. In vivo lipopolysaccharide exposure of human blood leukocytes induces cross-tolerance to multiple TLR ligands. J Immunol 183:533-42 (2009). WB ; Human . PubMed: 19542464