Anti-SARS spike glycoprotein antibody (ab22159)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab22159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: Use at a concentration of 0.1-1.0 µg/ml.
    WB: Use at a concentration of 0.1-1.0 µg/ml. Detects a band of approximately 139 kDa.
    [The antibody was tested on a cell line transfected with a partial-length cDNA coding for the N-terminal portion of SARS Spike protein with a predicted molecular weight of 68 kDa.]

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceA novel coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive stranded RNA approximately 27 to 31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2.
    • Alternative names
      • E2 antibody
      • E2 glycoprotein antibody
      • Human coronavirus spike glycoprotein antibody
      • S antibody
      • S glycoprotein antibody
      • Spike glycoprotein antibody
      see all

    Anti-SARS spike glycoprotein antibody images

    • All lanes : Anti-SARS spike glycoprotein antibody (ab22159) at 0.1 µg/ml

      Lane 1 : Transfected mouse melanoma cell lysate.
      Lane 2 : Untransfected mouse melanoma cell lysate.


      Observed band size : 68 kDa (why is the actual band size different from the predicted?)

    References for Anti-SARS spike glycoprotein antibody (ab22159)

    ab22159 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"