The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/1000. Predicted molecular weight: 58 kDa.
Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).
Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.
Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.
Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human hepatocarcinoma labeling SARS2 using ab175871 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot - Anti-SARS2 antibody (ab175871)
Anti-SARS2 antibody (ab175871) at 1/1000 dilution + HeLa cell lysate at 35 µg