Anti-SARS2 antibody - Aminoterminal end (ab71300)

Overview

  • Product name
    Anti-SARS2 antibody - Aminoterminal end
    See all SARS2 primary antibodies
  • Description
    Rabbit polyclonal to SARS2 - Aminoterminal end
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the N terminus of human SARS2 conjugated to KLH

  • Positive control
    • 293 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
WB 1/50 - 1/100. Detects a band of approximately 68 kDa (predicted molecular weight: 58 kDa).
IHC-P 1/10 - 1/50.

Target

  • Function
    Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).
  • Pathway
    Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; L-seryl-tRNA(Sec) from L-serine and tRNA(Sec): step 1/1.
  • Sequence similarities
    Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily.
  • Domain
    Consists of two distinct domains, a catalytic core and a N-terminal extension that is involved in tRNA binding.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • mitochondrial antibody
    • mtSerRS antibody
    • SARS antibody
    • SARS2 antibody
    • SARSM antibody
    • Serine tRNA ligase antibody
    • Serine--tRNA ligase antibody
    • SerRS antibody
    • SerRSmt antibody
    • SERS antibody
    • Seryl tRNA synthetase 2 antibody
    • Seryl-tRNA synthetase antibody
    • Seryl-tRNA(Ser/Sec) synthetase antibody
    • SYS antibody
    • SYSM_HUMAN antibody
    see all

Images

  • Anti-SARS2 antibody - Aminoterminal end (ab71300) at 1/60 dilution + 293 cell lysate at 35 µg

    Predicted band size : 58 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 54 kDa,72 kDa. We are unsure as to the identity of these extra bands.
  • ab71300 at 1/50 dilution staining SARS2 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

References

ab71300 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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