Overview

  • Product nameAnti-SART1 antibody
    See all SART1 primary antibodies
  • Description
    Mouse polyclonal to SART1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    full length human SART1, amino acids 1-800 (NP_005137.1).

  • Positive control
    • SART1 transfected 293T cell lysate, Jurkat cell lysate, HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab88583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 90 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • RelevanceSART1 encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri snRNP to the pre spliceosome in the spliceosome assembly pathway.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • Ara 1 antibody
    • ARA1 antibody
    • HOMS 1 antibody
    • HOMS1 antibody
    • hSART 1 antibody
    • hSART1 antibody
    • hSnu 66 antibody
    • hSnu66 antibody
    • IgE autoantigen antibody
    • MGC2038 antibody
    • SART 1 antibody
    • SART1 259 antibody
    • SART1(259) protein antibody
    • SART1(800) protein antibody
    • SART1259 antibody
    • Snu 66 antibody
    • Snu66 antibody
    • Squamous cell carcinoma antigen recognised by T cells antibody
    • Squamous cell carcinoma antigen recognized by T cells 1 antibody
    • U4/U6.U5 tri snRNP associated 110 kDa protein antibody
    • U4/U6.U5 tri snRNP associated protein 1 antibody
    see all

Anti-SART1 antibody images

  • Anti-SART1 antibody (ab88583) at 1 µg/ml + Jurkat cell lysate at 50 µg

    Secondary
    Goat anti-Mouse IgG (H+L) HRP at 1/50000 dilution

    Predicted band size : 90 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-SART1 antibody (ab88583) at 1 µg/ml

    Lane 1 : SART1 transfected 293T cell lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat anti-Mouse IgG (H+L) HRP at 1/5000 dilution

    Predicted band size : 90 kDa
    Observed band size : 130 kDa (why is the actual band size different from the predicted?)
  • ab88583 at 10µg/ml staining SART1 in HeLa cells by Immunofluorescence.

References for Anti-SART1 antibody (ab88583)

This product has been referenced in:
  • Taylor SE  et al. Differential sub-nuclear distribution of hypoxia-inducible factors (HIF)-1 and -2 alpha impacts on their stability and mobility. Open Biol 6:N/A (2016). ICC/IF ; Human . Read more (PubMed: 27655733) »
  • Laetsch TW  et al. Multiple components of the spliceosome regulate Mcl1 activity in neuroblastoma. Cell Death Dis 5:e1072 (2014). WB . Read more (PubMed: 24556687) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"