Recombinant
RabMAb

Anti-SART1 antibody [EPR14563(B)] (ab181867)

Overview

  • Product name
    Anti-SART1 antibody [EPR14563(B)]
    See all SART1 primary antibodies
  • Description
    Rabbit monoclonal [EPR14563(B)] to SART1
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SART1 aa 650-750. The exact sequence is proprietary.
    Database link: O43290

  • Positive control
    • HeLa, Jurkat and MOLT4 cell lysates; HeLa and Jurkat cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR14563(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 110 kDa (predicted molecular weight: 90 kDa).
ICC/IF 1/250.
Flow Cyt 1/150. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    SART1 encodes two proteins, the SART1(800) protein expressed in the nucleus of the majority of proliferating cells, and the SART1(259) protein expressed in the cytosol of epithelial cancers. The SART1(259) protein is translated by the mechanism of -1 frameshifting during posttranscriptional regulation; its full length sequence is not published yet. The two encoded proteins are thought to be involved in the regulation of proliferation. Both proteins have tumor rejection antigens. The SART1(259) protein possesses tumor epitopes capable of inducing HLA-A2402-restricted cytotoxic T lymphocytes in cancer patients. This SART1(259) antigen may be useful in specific immunotherapy for cancer patients and may serve as a paradigmatic tool for the diagnosis and treatment of patients with atopy. The SART1(259) protein is found to be essential for the recruitment of the tri snRNP to the pre spliceosome in the spliceosome assembly pathway.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • Ara 1 antibody
    • ARA1 antibody
    • HOMS 1 antibody
    • HOMS1 antibody
    • hSART 1 antibody
    • hSART1 antibody
    • hSnu 66 antibody
    • hSnu66 antibody
    • IgE autoantigen antibody
    • MGC2038 antibody
    • SART 1 antibody
    • SART1 259 antibody
    • SART1(259) protein antibody
    • SART1(800) protein antibody
    • SART1259 antibody
    • Snu 66 antibody
    • Snu66 antibody
    • Squamous cell carcinoma antigen recognised by T cells antibody
    • Squamous cell carcinoma antigen recognized by T cells 1 antibody
    • U4/U6.U5 tri snRNP associated 110 kDa protein antibody
    • U4/U6.U5 tri snRNP associated protein 1 antibody
    see all

Images

  • All lanes : Anti-SART1 antibody [EPR14563(B)] (ab181867) at 1/50000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : MOLT4 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 90 kDa
  • Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling SART1 with ab181867 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Flow cytometric analysis of Jurkat cells (paraformaldehyde-fixed, 2%) labeling SART1 with ab181867 at 1/150 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

References

ab181867 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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