Overview

  • Product name
    Anti-SART3 antibody - C-terminal
    See all SART3 primary antibodies
  • Description
    Rabbit polyclonal to SART3 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within C terminal amino acids 901-963 of Human SART3 (Uniprot ID: Q15020).

  • Positive control
    • A431, H1299, HeLa, HepG2 and Raji whole cell lysates; HeLa cells; Human NCI-N87 xenograft tissue.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab155765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 110 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Regulates Tat transactivation activity through direct interaction. May be a cellular factor for HIV-1 gene expression and viral replication.
  • Tissue specificity
    Ubiquitously expressed.
  • Involvement in disease
    Defects in SART3 are the cause of disseminated superficial actinic porokeratosis type 1 (DSAP1) [MIM:175900]. DSAP1 is an autosomal dominant disorder, characterized by multiple superficial keratotic lesions surrounded by a slightly raised keratotic border, developing during the third or fourth decade of life on sun-exposed areas of skin.
  • Sequence similarities
    Contains 8 HAT repeats.
    Contains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus speckle. Localized in speckles. Expressed in the nucleus of all of the malignant tumor cell lines tested and the majority of cancer tissues with various histologies, including squamous cell carcinomas (SCC), adenocarcinomas, melanomas and leukemias cells. However, this protein is undetectable in the nucleus of any cell lines of nonmalignant cells or normal tissues, except for the testis. Expressed in the cytoplasm of all the proliferating cells, including normal and malignant cells, but not in normal tissues, except for the testis and the fetal liver.
  • Information by UniProt
  • Database links
  • Alternative names
    • DSAP1 antibody
    • hSART 3 antibody
    • hSART-3 antibody
    • P100 antibody
    • P110 antibody
    • p110(nrb) antibody
    • RP11 13G14 antibody
    • SART 3 antibody
    • SART-3 antibody
    • SART3 antibody
    • SART3_HUMAN antibody
    • Squamous cell carcinoma antigen recognized by T cells 3 antibody
    • Squamous cell carcinoma antigen recognized by T-cells 3 antibody
    • Tat interacting protein of 110 kDa antibody
    • Tat-interacting protein of 110 kDa antibody
    • Tip110 antibody
    see all

Images

  • All lanes : Anti-SART3 antibody - C-terminal (ab155765) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate
    Lane 3 : HeLa whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 110 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling SART3 with ab155765 at 1/200 dilution. Lower image is merged with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded Human NCI-N87 xenograft tissue labeling SART3 with ab155765 at 1/100 dilution.

References

ab155765 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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