Overview

  • Product name
    Anti-SASS6 antibody
  • Description
    Rabbit polyclonal to SASS6
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 607 and 657 of Human SASS6 (NP_919268.1).

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab85993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Relevance
    SASS6 (Spindle assembly abnormal protein 6 homolog) is required for centrosome duplication. It functions to ensure that each centriole seeds the formation of a single procentriole per cell cycle. Overexpression results in excess foci bearing centriolar markers.
  • Cellular localization
    Cytoplasm; cytoskeleton; centrosome. Cytoplasm; cytoskeleton; centrosome; centriole. Note: Component of the centrosome. Associated only transiently with nascent procentrioles during centriole biogenesis.
  • Database links
  • Alternative names
    • 2810453L12Rik antibody
    • FLJ22097 antibody
    • HsSAS 6 antibody
    • HsSAS6 antibody
    • MGC119440 antibody
    • RGD1306255 antibody
    • SAS 6 antibody
    • SAS6 antibody
    • SASS 6 antibody
    • Spindle assembly 6 homolog (C. elegans) antibody
    • Spindle assembly 6 homolog antibody
    • Spindle assembly abnormal protein 6 antibody
    • Spindle assembly abnormal protein 6 homolog antibody
    see all

Images

  • All lanes : Anti-SASS6 antibody (ab85993) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 74 kDa
    Observed band size : 74 kDa


    Exposure time : 3 minutes
  • Detection of Human SASS6 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85993 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab85993 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab85993.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up