Overview

  • Product nameAnti-SASS6 antibody
    See all SASS6 primary antibodies
  • Description
    Rabbit polyclonal to SASS6
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residues 607 and 657 of Human SASS6 (NP_919268.1).

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab85993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • RelevanceSASS6 (Spindle assembly abnormal protein 6 homolog) is required for centrosome duplication. It functions to ensure that each centriole seeds the formation of a single procentriole per cell cycle. Overexpression results in excess foci bearing centriolar markers.
  • Cellular localizationCytoplasm; cytoskeleton; centrosome. Cytoplasm; cytoskeleton; centrosome; centriole. Note: Component of the centrosome. Associated only transiently with nascent procentrioles during centriole biogenesis.
  • Database links
  • Alternative names
    • 2810453L12Rik antibody
    • FLJ22097 antibody
    • HsSAS 6 antibody
    • HsSAS6 antibody
    • MGC119440 antibody
    • RGD1306255 antibody
    • SAS 6 antibody
    • SAS6 antibody
    • SASS 6 antibody
    • Spindle assembly 6 homolog (C. elegans) antibody
    • Spindle assembly 6 homolog antibody
    • Spindle assembly abnormal protein 6 antibody
    • Spindle assembly abnormal protein 6 homolog antibody
    see all

Anti-SASS6 antibody images

  • All lanes : Anti-SASS6 antibody (ab85993) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 74 kDa
    Observed band size : 74 kDa


    Exposure time : 3 minutes
  • Detection of Human SASS6 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab85993 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.

References for Anti-SASS6 antibody (ab85993)

ab85993 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab85993.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"