Overview

  • Product nameAnti-SAT1 antibody
  • Description
    Rabbit polyclonal to SAT1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Pig, Macaque Monkey, Gorilla, Chinese Hamster
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human SAT1.

  • Positive control
    • This antibody gave a positive signal in Human Adipose tissue lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).

Target

  • FunctionEnzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine = spermidine >> spermine > N(1)-acetylspermine > putrescine. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Also involved in the regulation of polyamine transport out of cells. Acts on 1,3-diaminopropane, 1,5-diaminopentane, putrescine, spermidine (forming N(1)- and N(8)-acetylspermidine), spermine, N(1)-acetylspermidine and N(8)-acetylspermidine.
  • PathwayAmine and polyamine degradation; putrescine degradation; N-acetylputrescine from putrescine: step 1/1.
  • Involvement in diseaseKeratosis follicularis spinulosa decalvans X-linked
  • Sequence similaritiesBelongs to the acetyltransferase family.
    Contains 1 N-acetyltransferase domain.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • DC21 antibody
    • Diamine acetyltransferase 1 antibody
    • Diamine N acetyltransferase 1 antibody
    • EC 2.3.1.57 antibody
    • KFSD antibody
    • KFSDX antibody
    • Polyamine N acetyltransferase 1 antibody
    • Polyamine N-acetyltransferase 1 antibody
    • Putrescine acetyltransferase antibody
    • SAT antibody
    • SAT1 antibody
    • SAT1_HUMAN antibody
    • Spermidine/spermine N(1) acetyltransferase 1 antibody
    • Spermidine/spermine N(1)-acetyltransferase 1 antibody
    • Spermidine/spermine N1 acetyltransferase 1 antibody
    • Spermidine/spermine N1 acetyltransferase alpha antibody
    • SSAT 1 antibody
    • SSAT antibody
    • SSAT-1 antibody
    see all

Anti-SAT1 antibody images

  • Anti-SAT1 antibody (ab105220) at 1 µg/ml + Human adipose normal tissue lysate - total protein (ab28980) at 25 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 20 kDa
    Observed band size : 20 kDa
    Additional bands at : 110 kDa,39 kDa,60 kDa,78 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

References for Anti-SAT1 antibody (ab105220)

ab105220 has not yet been referenced specifically in any publications.

Product Wall

Application IHC - Wholemount
Sample Human Tissue (Mouse Brain injected with human neurospheres)
Specification Mouse Brain injected with human neurospheres
Username

Adina Brett-Morris

Verified customer

Submitted Jan 27 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"