Anti-SAT1 antibody (ab105220)
Key features and details
- Rabbit polyclonal to SAT1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SAT1 antibody
See all SAT1 primary antibodies -
Description
Rabbit polyclonal to SAT1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Pig, Macaque monkey, Gorilla, Chinese hamster -
Immunogen
Synthetic peptide corresponding to Human SAT1 aa 100 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab127967) -
Positive control
- Recombinant Human SAT1 protein (ab87666) can be used as a positive control in WB. This antibody gave a positive signal in Human Adipose tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab105220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). |
Target
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Function
Enzyme which catalyzes the acetylation of polyamines. Substrate specificity: norspermidine = spermidine >> spermine > N(1)-acetylspermine > putrescine. This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines. Also involved in the regulation of polyamine transport out of cells. Acts on 1,3-diaminopropane, 1,5-diaminopentane, putrescine, spermidine (forming N(1)- and N(8)-acetylspermidine), spermine, N(1)-acetylspermidine and N(8)-acetylspermidine. -
Pathway
Amine and polyamine degradation; putrescine degradation; N-acetylputrescine from putrescine: step 1/1. -
Involvement in disease
Keratosis follicularis spinulosa decalvans X-linked -
Sequence similarities
Belongs to the acetyltransferase family.
Contains 1 N-acetyltransferase domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 100689427 Chinese hamster
- Entrez Gene: 508861 Cow
- Entrez Gene: 6303 Human
- Entrez Gene: 20229 Mouse
- Entrez Gene: 397645 Pig
- Entrez Gene: 302642 Rat
- Omim: 313020 Human
- SwissProt: Q9JHW6 Chinese hamster
see all -
Alternative names
- DC21 antibody
- Diamine acetyltransferase 1 antibody
- Diamine N acetyltransferase 1 antibody
see all
Images
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Anti-SAT1 antibody (ab105220) at 1 µg/ml + Human adipose normal tissue lysate - total protein (ab28980) at 25 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 20 kDa
Additional bands at: 110 kDa, 39 kDa, 60 kDa, 78 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutes
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab105220 has been referenced in 7 publications.
- Zhang X et al. Exogenous spermine attenuates diabetic kidney injury in rats by inhibiting AMPK/mTOR signaling pathway. Int J Mol Med 47:N/A (2021). PubMed: 33537831
- Sandusky-Beltran LA et al. Aberrant AZIN2 and polyamine metabolism precipitates tau neuropathology. J Clin Invest 131:N/A (2021). PubMed: 33586680
- Wang Z et al. CREB stimulates GPX4 transcription to inhibit ferroptosis in lung adenocarcinoma. Oncol Rep 45:N/A (2021). PubMed: 33846793
- Thakur VS & Welford SM Generation of a conditional mutant knock-in under the control of the natural promoter using CRISPR-Cas9 and Cre-Lox systems. PLoS One 15:e0240256 (2020). PubMed: 33007045
- Xiao Y et al. LINC00265 targets miR-382-5p to regulate SAT1, VAV3 and angiogenesis in osteosarcoma. Aging (Albany NY) 12:20212-20225 (2020). PubMed: 33109774
- Tamari K et al. Polyamine flux suppresses histone lysine demethylases and enhances ID1 expression in cancer stem cells. Cell Death Discov 4:104 (2018). PubMed: 30455990
- Li X et al. LC-MS-based metabolomics revealed SLC25A22 as an essential regulator of aspartate-derived amino acids and polyamines inKRAS-mutant colorectal cancer. Oncotarget 8:101333-101344 (2017). PubMed: 29254168