Overview

  • Product nameAnti-SATB1 antibody
    See all SATB1 primary antibodies
  • Description
    Rabbit polyclonal to SATB1
  • SpecificityThis antibody is specific for SATB1
  • Tested applicationsSuitable for: ELISA, IP, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cat
  • Immunogen

    A region within synthetic peptide: DVAEYKEEEL LKDLEESVQD KNTNTLFSVK LEEELSVEGN TDINTDLKD, corresponding to C terminal amino acids 715-763 of Human SATB1

  • Positive control
    • Jurkat cell lysate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab49061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 86 kDa).Can be blocked with Human SATB1 peptide (ab111669). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100.

Target

  • FunctionCrucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1-binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly upregulating metastasis-associated genes while downregulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis.
  • Tissue specificityExpressed predominantly in thymus.
  • Sequence similaritiesBelongs to the CUT homeobox family.
    Contains 2 CUT DNA-binding domains.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications
    Sumoylated. Sumoylation promotes cleavage by caspases.
    Phosphorylated by PKC. Acetylated by PCAF. Phosphorylated form interacts with HDAC1, but unphosphorylated form interacts with PCAF. DNA binding properties are activated by phosphorylation and inactivated by acetylation. In opposition, gene expression is down-regulated by phosphorylation but up-regulated by acetylation.
    Cleaved at Asp-254 by caspase-3 and caspase-6 during T-cell apoptosis in thymus and during B-cell stimulation. The cleaved forms can not dimerize and lose transcription regulation function because of impaired DNA and chromatin association.
  • Cellular localizationNucleus matrix. Nucleus > PML body. Organized into a cage-like network anchoring loops of heterochromatin and tethering specialized DNA sequences. When sumoylated, localized in promyelocytic leukemia nuclear bodies.
  • Information by UniProt
  • Database links
  • FormThere are 2 isoforms produced by alternative splicing.
  • Alternative names
    • DNA binding protein SATB1 antibody
    • DNA-binding protein SATB1 antibody
    • SATB homeobox 1 antibody
    • SATB1 antibody
    • SATB1_HUMAN antibody
    • Special AT rich sequence binding protein 1 (binds to nuclear matrix/scaffold associating DNA) antibody
    • Special AT rich sequence binding protein 1 antibody
    • Special AT-rich sequence-binding protein 1 antibody
    see all

Anti-SATB1 antibody images

  • Lane 1 : Anti-SATB1 antibody (ab49061) at 1.25 µg/ml
    Lane 2 : Anti-SATB1 antibody (ab49061) at 2.5 µg/ml
    Lane 3 : Anti-SATB1 antibody (ab49061) at 5 µg/ml

    Lane 1 : Cell lysate prepared from human Jurkat cells
    Lane 2 : Cell lysate prepared from human Jurkat cells
    Lane 3 : Cell lysate prepared from human Jurkat cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 86 kDa
  • ab49061 staining SATB1 in HEK 293T cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed with formaldehyde, permeabilized using 0.1 % Triton X-100 for 5 minutes, blocked with 2% BSA for 1 hour at 25°C and then incubated with ab49061 at a 1/100 dilution for 3 hours at 25°C. The secondary used was an Oregon Green 488 conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.

    See Abreview

  • Estrogen Related Receptor gamma was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to Estrogen Related Receptor gamma and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab49061.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 100KDa: Estrogen Related Receptor gamma

References for Anti-SATB1 antibody (ab49061)

This product has been referenced in:
  • Shukla S  et al. Upregulation of SATB1 is associated with prostate cancer aggressiveness and disease progression. PLoS One 8:e53527 (2013). WB ; Human . Read more (PubMed: 23308245) »
  • Balamotis MA  et al. Satb1 ablation alters temporal expression of immediate early genes and reduces dendritic spine density during postnatal brain development. Mol Cell Biol 32:333-47 (2012). WB, ICC/IF ; Mouse . Read more (PubMed: 22064485) »

See all 3 Publications for this product

Product Wall

Thank you for your reply.

To my knowledge, the positive control used in our western blot was not activated Jurkat cell lysate. There is a Jurkat lysate available in our catalogue:

http://www.abcam.com/Jurkat-Human-T-cell-lymphoblast...

Read More

Thank you for contacting us.

I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

We have used in our lab Jurkat cell lysates with successful results with 1/800 dilution, as shown in our data...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HEK 293T cells)
Specification HEK 293T cells
Fixative Formaldehyde
Permeabilization Yes - 0.1 % TritonX-100 for 5 minutes
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK 293T and HeLa cells)
Loading amount 30 µg
Specification HEK 293T and HeLa cells
Gel Running Conditions Reduced Denaturing (7.5% PAGE Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Ms. Shilpa Agarawal

Verified customer

Submitted Apr 27 2012

Thank you for contacting us. We added IHC -P to the datasheet based on a reference that used the antibody for this application: Agrelo Ret al. SATB1 defines the developmental context for gene silencing by Xist in lymphoma and embryonic cells. Dev...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293T cells grown in 5%CO2 at 37 degrees, in DME)
Loading amount 20 µg
Specification HEK293T cells grown in 5%CO2 at 37 degrees, in DME
Gel Running Conditions Reduced Denaturing (10 % SDS-PAGE gel ran in 1X Tris-Glycine-SDS)
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 28 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (skin)
Specification skin
Fixative Formaldehyde
Permeabilization No
Username

Dr. Andrei Mardaryev

Verified customer

Submitted Aug 19 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"