Anti-SATB1 antibody (ab70004)
Key features and details
- Rabbit polyclonal to SATB1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SATB1 antibody
See all SATB1 primary antibodies -
Description
Rabbit polyclonal to SATB1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
18 amino acid peptide near the amino terminus of the human SATB1 (GenBank accession no. Q01826).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 100, 110 kDa (predicted molecular weight: 85 kDa).
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ICC/IF |
Use a concentration of 20 µg/ml.
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IHC-P |
Use a concentration of 2.5 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 100, 110 kDa (predicted molecular weight: 85 kDa). |
ICC/IF
Use a concentration of 20 µg/ml. |
IHC-P
Use a concentration of 2.5 µg/ml. |
Target
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Function
Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1-binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly upregulating metastasis-associated genes while downregulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis. -
Tissue specificity
Expressed predominantly in thymus. -
Sequence similarities
Belongs to the CUT homeobox family.
Contains 2 CUT DNA-binding domains.
Contains 1 homeobox DNA-binding domain. -
Post-translational
modificationsSumoylated. Sumoylation promotes cleavage by caspases.
Phosphorylated by PKC. Acetylated by PCAF. Phosphorylated form interacts with HDAC1, but unphosphorylated form interacts with PCAF. DNA binding properties are activated by phosphorylation and inactivated by acetylation. In opposition, gene expression is down-regulated by phosphorylation but up-regulated by acetylation.
Cleaved at Asp-254 by caspase-3 and caspase-6 during T-cell apoptosis in thymus and during B-cell stimulation. The cleaved forms can not dimerize and lose transcription regulation function because of impaired DNA and chromatin association. -
Cellular localization
Nucleus matrix. Nucleus > PML body. Organized into a cage-like network anchoring loops of heterochromatin and tethering specialized DNA sequences. When sumoylated, localized in promyelocytic leukemia nuclear bodies. - Information by UniProt
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Database links
- Entrez Gene: 6304 Human
- Omim: 602075 Human
- SwissProt: Q01826 Human
- Unigene: 517717 Human
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Form
There are 2 isoforms produced by alternative splicing. -
Alternative names
- DNA binding protein SATB1 antibody
- DNA-binding protein SATB1 antibody
- SATB homeobox 1 antibody
see all
Images
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Lane 1 : Anti-SATB1 antibody (ab70004) at 1 µg/ml
Lane 2 : Anti-SATB1 antibody (ab70004) at 2 µg/ml
All lanes : SK-N-SH cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 85 kDa
Additional bands at: 100 kDa (possible isoform), 110 kDa (possible isoform) -
ab70004 at 2.5µg/ml staining SATB1 in human brain tissue section by immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
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Immunofluorescence of SATB1 in Human Brain cells using ab70004 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab70004 has been referenced in 14 publications.
- Ruff T et al. FLRT3 Marks Direction-Selective Retinal Ganglion Cells That Project to the Medial Terminal Nucleus. Front Mol Neurosci 14:790466 (2021). PubMed: 34955746
- Seo W et al. Runx-mediated regulation of CCL5 via antagonizing two enhancers influences immune cell function and anti-tumor immunity. Nat Commun 11:1562 (2020). PubMed: 32218434
- Yin CY et al. miR-7-5p inhibits cell migration and invasion in glioblastoma through targeting SATB1. Oncol Lett 17:1819-1825 (2019). PubMed: 30675243
- Yasuda K et al. Satb1 regulates the effector program of encephalitogenic tissue Th17 cells in chronic inflammation. Nat Commun 10:549 (2019). PubMed: 30710091
- Salib M et al. GABAergic Medial Septal Neurons with Low-Rhythmic Firing Innervating the Dentate Gyrus and Hippocampal Area CA3. J Neurosci 39:4527-4549 (2019). PubMed: 30926750