Validated using a knockout cell line

Anti-SATB1 antibody [EPR3951] (ab109122)


  • Product name
    Anti-SATB1 antibody [EPR3951]
    See all SATB1 primary antibodies
  • Description
    Rabbit monoclonal [EPR3951] to SATB1
  • Host species
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC, IHC-Frmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human SATB1. The exact sequence is proprietary.

  • Positive control
    • Jurkat, fetal thymus, and Mouse thymus cell lysates; Human breast carcinoma, tonsil and ovarian adenocarcinoma tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab109122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 86 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC 1/100 - 1/250.
IHC-Fr Use at an assay dependent concentration. PubMed: 22123820
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Crucial silencing factor contributing to the initiation of X inactivation mediated by Xist RNA that occurs during embryogenesis and in lymphoma (By similarity). Binds to DNA at special AT-rich sequences, the consensus SATB1-binding sequence (CSBS), at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcriptional repressor controlling nuclear and viral gene expression in a phosphorylated and acetylated status-dependent manner, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes (e.g. PML at the MHC-I locus) and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Modulates genes that are essential in the maturation of the immune T-cell CD8SP from thymocytes. Required for the switching of fetal globin species, and beta- and gamma-globin genes regulation during erythroid differentiation. Plays a role in chromatin organization and nuclear architecture during apoptosis. Interacts with the unique region (UR) of cytomegalovirus (CMV). Alu-like motifs and SATB1-binding sites provide a unique chromatin context which seems preferentially targeted by the HIV-1 integration machinery. Moreover, HIV-1 Tat may overcome SATB1-mediated repression of IL2 and IL2RA (interleukin) in T-cells by binding to the same domain than HDAC1. Delineates specific epigenetic modifications at target gene loci, directly upregulating metastasis-associated genes while downregulating tumor-suppressor genes. Reprograms chromatin organization and the transcription profiles of breast tumors to promote growth and metastasis.
    • Tissue specificity
      Expressed predominantly in thymus.
    • Sequence similarities
      Belongs to the CUT homeobox family.
      Contains 2 CUT DNA-binding domains.
      Contains 1 homeobox DNA-binding domain.
    • Post-translational
      Sumoylated. Sumoylation promotes cleavage by caspases.
      Phosphorylated by PKC. Acetylated by PCAF. Phosphorylated form interacts with HDAC1, but unphosphorylated form interacts with PCAF. DNA binding properties are activated by phosphorylation and inactivated by acetylation. In opposition, gene expression is down-regulated by phosphorylation but up-regulated by acetylation.
      Cleaved at Asp-254 by caspase-3 and caspase-6 during T-cell apoptosis in thymus and during B-cell stimulation. The cleaved forms can not dimerize and lose transcription regulation function because of impaired DNA and chromatin association.
    • Cellular localization
      Nucleus matrix. Nucleus > PML body. Organized into a cage-like network anchoring loops of heterochromatin and tethering specialized DNA sequences. When sumoylated, localized in promyelocytic leukemia nuclear bodies.
    • Information by UniProt
    • Database links
    • Form
      There are 2 isoforms produced by alternative splicing.
    • Alternative names
      • DNA binding protein SATB1 antibody
      • DNA-binding protein SATB1 antibody
      • SATB homeobox 1 antibody
      • SATB1 antibody
      • SATB1_HUMAN antibody
      • Special AT rich sequence binding protein 1 (binds to nuclear matrix/scaffold associating DNA) antibody
      • Special AT rich sequence binding protein 1 antibody
      • Special AT-rich sequence-binding protein 1 antibody
      see all


    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: SATB1 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: Jurkat whole cell lysate (20 µg)
      Lane 4: THP1 whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab109122 observed at 100 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab109122 was shown to specifically react with SATB1 in wild-type HAP1 cells as signal was lost in SATB1 knockout cells. Wild-type and SATB1 knockout samples were subjected to SDS-PAGE. Ab109122 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-SATB1 antibody [EPR3951] (ab109122) at 1/1000 dilution

      Lane 1 : Jurkat cell lysates
      Lane 2 : Fetal thymus lysates
      Lane 3 : Mouse thymus lysates

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size: 86 kDa

    • ab109122, at 1/100 dilution staining SATB1 in paraffin-embedded Human breast carcinoma, by Immunohistochemistry.
    • ab109122 staining SATB1 in mouse skin tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue samples were fixed with paraformaldehyde, blocked with 10% goat serum for 30 minutes at 22°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/50 in PBS) at 4°C for 12 hours. A FITC-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

      See Abreview

    • ab109122, at 1/100 dilution staining SATB1 in paraffin-embedded Human ovarian adenocarcinoma tissue, by Immunohistochemistry.
    • ab109122, at 1/100 dilution staining SATB1 in paraffin-embedded Human tonsil tissue, by Immunohistochemistry.


    This product has been referenced in:
    • Gong J  et al. Hepatic SATB1 induces paracrine activation of hepatic stellate cells and is upregulated by HBx. Sci Rep 6:37717 (2016). IHC-P ; Mouse . Read more (PubMed: 27883059) »
    • Hao B  et al. An anti-silencer- and SATB1-dependent chromatin hub regulates Rag1 and Rag2 gene expression during thymocyte development. J Exp Med 212:809-24 (2015). CHIPseq ; Mouse . Read more (PubMed: 25847946) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Mouse Tissue lysate - nuclear (Thymus)
    Negative control
    upstream element of Gata6 all the procedures were done in wild type and in Satb1 knockout cells
    Detection step
    Real-time PCR
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 10 minute(s) and 0 second(s)
    Specification of the cross-linking agent: formaldehyde
    Positive control
    RHS6 region (previously worked in our laboratory)

    Abcam user community

    Verified customer

    Submitted Jul 11 2016

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Mouse Tissue sections (Skin)
    Antigen retrieval step
    Heat mediated
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C

    Ahmar Aziz

    Verified customer

    Submitted Dec 16 2015


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