Overview

  • Product name
    Anti-SATB2 antibody
    See all SATB2 primary antibodies
  • Description
    Rabbit polyclonal to SATB2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, EMSA, IHC-FoFr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Mouse SATB2.

    Read Abcam's proprietary immunogen policy

    (Peptide available as ab34906.)

  • Positive control
    • Brain (Mouse) Whole Cell Lysate - normal tissue, 0 days old.

Properties

Applications

Our Abpromise guarantee covers the use of ab34735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 83 kDa).
EMSA Use at an assay dependent concentration. PubMed: 22123820
IHC-FoFr Use at an assay dependent concentration. PubMed: 19709629
ICC/IF Use at an assay dependent concentration. PubMed: 19232401

Target

  • Function
    Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.
  • Tissue specificity
    High expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.
  • Involvement in disease
    Note=Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11).
    Defects in SATB2 are a cause of cleft palate isolated (CPI) [MIM:119540]. A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis.
    Note=A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22).
  • Sequence similarities
    Belongs to the CUT homeobox family.
    Contains 2 CUT DNA-binding domains.
    Contains 1 homeobox DNA-binding domain.
  • Post-translational
    modifications
    Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity.
  • Cellular localization
    Nucleus matrix.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • DNA binding protein SATB2 antibody
    • DNA-binding protein SATB2 antibody
    • FLJ21474 antibody
    • FLJ32076 antibody
    • GLSS antibody
    • KIAA1034 antibody
    • MGC119474 antibody
    • MGC119477 antibody
    • SATB family member 2 antibody
    • SATB homeobox 2 antibody
    • SATB2 antibody
    • SATB2_HUMAN antibody
    • Special AT rich sequence binding protein 2 antibody
    • Special AT-rich sequence-binding protein 2 antibody
    see all

Images

  • Western blot - SATB2 antibody (ab34735)
    Western blot - SATB2 antibody (ab34735)
    Anti-SATB2 antibody (ab34735) at 1 µg/ml + Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

    Performed under reducing conditions.

    Predicted band size: 83 kDa
    Observed band size: 85 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands.

  • Electrophoretic Mobility Shift Assay - Anti-SATB2 antibody (ab34735)
    Electrophoretic Mobility Shift Assay - Anti-SATB2 antibody (ab34735)Image from Asanoma K et al, J Biol Chem. 2012 Jan 13;287(3):2257-68. Epub 2011 Nov 28, Fig 8. DOI 10.1074/jbc.M111.287128 January 13, 2012 The Journal of Biological Chemistry, 287, 2257-2268.
    ab34735 used in EMSA.
    EMSA demonstrating binding of SATB1 and SATB2 to an oligonucleotide containing a predicted SATB-binding element located 125 bp upstream of the Eomes transcription start site (-125). Labeled SATB probes were incubated with Rcho-1 TS cell nuclear lysates as indicated above the lanes. Lane 1, without any competitor oligonucleotide; lane 2, with unlabeled wild-type competitor; lane 3, with unlabeled mutant competitor; lane 4, with SATB1 antibody; lane 5, with SATB2 antibody (ab34735); lane 6, with a control antibody. B and C, Rcho-1 TS cells stably transfected with pcDNA3, pcDNA3-HA-Satb1, or pcDNA3-HA-Satb2 and maintained in stem culture conditions were used for ChIP assays. Antibodies to the HA tag (Anti-HA) or acetylated histone H3K9 (Anti-AcH3) were used in the analyses. IgG was used as a control for nonspecific immunoprecipitation. qPCR analyses were performed on immunoprecipitates using primers amplifying the segment (-172 to -63) including the SATB-binding element at -125.
  • Immunocytochemistry/ Immunofluorescence - Anti-SATB2 antibody (ab34735)
    Immunocytochemistry/ Immunofluorescence - Anti-SATB2 antibody (ab34735)Image courtesy of an anonymous Abreview.
    ab34735 staining SATB2 in human osteosarcoma SAOS-2 cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in paraformaldehyde, permeabilized using PBS/ 0.25% Triton, blocked with 1% BSA for 1 hour at room temperature and then incubated with ab34735 at a 1/500 dilution for 1 hour. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/250 dilution.

    See Abreview

References

This product has been referenced in:
  • Sahu SK  et al. FBXO32 promotes microenvironment underlying epithelial-mesenchymal transition via CtBP1 during tumour metastasis and brain development. Nat Commun 8:1523 (2017). Read more (PubMed: 29142217) »
  • Wang Y  et al. LSD1 co-repressor Rcor2 orchestrates neurogenesis in the developing mouse brain. Nat Commun 7:10481 (2016). IHC (PFA fixed) ; Mouse . Read more (PubMed: 26795843) »

See all 20 Publications for this product

Publishing research using ab34735? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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10 reviews or Q&A

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Votes: Highest First

I’ve been recently interested greatly with SATB2 protein as a potential target for cleavage by a specific protease. I’ve been able to show interesting western blot results using the Abcam SATB2 antibody (ab34735), however when I went on the...

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Thank you for contacting Abcam.

The immunogen sequence for ab34735 is ********************************.

I hope that your future experiments go well and please let me know if there is anything else I can help you with.

Immunocytochemistry/ Immunofluorescence abreview for Anti-SATB2 antibody

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human osteosarcoma SAOS-2 cell line)
Specification
human osteosarcoma SAOS-2 cell line
Fixative
Paraformaldehyde
Permeabilization
Yes - PBS-TRITON 0.25%
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
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Verified customer

Submitted Apr 19 2012

Here’s a quick review of what I do:



Cells tested: C2C12 myoblasts AND primary mouse myoblasts



Dilutions & Times:

SATB2 primary Ab (abcam) 1:2500 in 5% Milk-TTBS Overnight at 4C rotating...

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Thank you for providing the details of your protocol.

Have you tried using the primary antibody at a 1:1000 dilution? Because this is a polyclonal antibody, there may be some batch to batch variability. Our internal QC will only pass this ant...

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My email is regarding Abcam primary antibody for SATB2 (rabbit polycolonal) with Cat # ab34735

I have purchased this antibody body (as well as other antibodies from your extensive catalogue) and it worked well showing the desired ˜85kDa band...

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Thank you for contacting us. I am sorry to hear that this batch is not working for your western blot. It is certainly possible that there may be a batch specific issue with the lot you received. If that is the case and you have purchased the antibody i...

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Please send a testing discount code for testing human reactivity by western blotting.

DISCOUNT CODE: ******* Expiration date: ******** I am very pleased to hear you would like to accept our offer and test ab34735 on human samples. This code will give you one free primary antibody before the expiration date. To redeem this offer, please ...

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Western blot abreview for Anti-SATB2 antibody

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (NT2D1 cells)
Loading amount
10 µg
Specification
NT2D1 cells
Gel Running Conditions
Reduced Denaturing (10% gel, SDS buffer used for running in BioRad Protean tetracell)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Jan 03 2012

I dread doing a 4th western with this antibody- if you can send a new vial to my address here that would be great.  It was originally purchased by my collaborator at AFFRI (Dr. Alexandra Miller) and mailed to her.  Is there any way you can mail it to m...

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I received an email today from your collaborator giving permission for the replacement antibody to be sent to you. I have processed your request to have ab51502 sent to you directly.

I dread doing a 4th western with this antibody- if you can send a new vial to my address here that would be great.  It was originally purchased by my collaborator and mailed to her.  Is there any way you can mail it to me directly?  If not, she'll be a...

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As we just discussed on the phone, to process you free of charge replacement, I do not confirmation from your collaborator that it is ok for me to send the new antibody to you. Once I receive the confirmation, I will have the new antibody you requeste...

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I don't think the problem can be degradation, since two of the bands are higher than the expected 85kDa band.

Looking the images you sent, the blot on the bottom right, shows a band at approximately 75kDa and a band below 100kDa, could this be the 85kDa band? There is also a band just above that. Does SATB2 undergo any post-translational modifications that cou...

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Here's a brief summary of what I did so far: 1st attempt: no signal at all (1:1000) 2nd attempt, at Tom's recommendation tried 1% BSA, and the whole membrane was black (on the film) (1:500) 3rd attempt, described below, with photos attached From left t...

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As the images are black and white, I am unable to determine which band is the 75kDa pink band you mention in your methods. Could you send me a labeled version of the blot, so that I can see where the band of interest should be. In general though, yo...

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