You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-SATB2 antibody [SATBA4B10] (ab51502)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-SATB2 antibody [SATBA4B10]
See all SATB2 products (6) ...

Description

Mouse monoclonal [SATBA4B10] to SATB2

Tested applications

IP, ICC/IF, IHC-FoFr, WB, IHC-Fr, IHC-Pmore details

Cross reactivity

Reacts with

Mouse, Rat, Human, Zebrafish

Immunogen

Recombinant fragment C-terminal. (Human)

Positive control

HT-1080 whole cell lysate. RIPA lysateof HeLacells. HeLa cells. NIH3T3 whole cell lysate.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, 8mM Dibasic monohydrogen sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Monobasic dihydrogen potassium phosphate, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

Concentrated and dialyzed culture medium (5% FBS medium without bovine IgG(<95%approximately)) of hybridoma Purified by ProteinG Filtered through a 0.22 µm membrane and packed aseptically.

Clonality

Monoclonal

Clone number

SATBA4B10

Isotype

IgG1

Research areas

Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Neuroscience >> Neurology process >> Neurogenesis

  • Western blot - SATB2 antibody [SATBA4B10] (ab51502)Western blot - SATB2 antibody [SATBA4B10] (ab51502) image (enlarge)

  • Immunoprecipitation - SATB2 antibody [SATBA4B10] (ab51502)Immunoprecipitation - SATB2 antibody [SATBA4B10] (ab51502) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - SATB2 antibody [SATBA4B10] (ab51502)Immunocytochemistry/ Immunofluorescence - SATB2 antibody [SATBA4B10] (ab51502) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab51502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: 1/5.
IHC-P: 1/400. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: 1/50 - 1/400.
IHC-FoFr: Use at an assay dependent dilution (PMID 19864583).
IP: 1/100.
WB: 1/100. Predicted molecular weight: 81 kDa.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Binds to DNA, at nuclear matrix- or scaffold-associated regions. Thought to recognize the sugar-phosphate structure of double-stranded DNA. Transcription factor controlling nuclear gene expression, by binding to matrix attachment regions (MARs) of DNA and inducing a local chromatin-loop remodeling. Acts as a docking site for several chromatin remodeling enzymes and also by recruiting corepressors (HDACs) or coactivators (HATs) directly to promoters and enhancers. Required for the initiation of the upper-layer neurons (UL1) specific genetic program and for the inactivation of deep-layer neurons (DL) and UL2 specific genes, probably by modulating BCL11B expression. Repressor of Ctip2 and regulatory determinant of corticocortical connections in the developing cerebral cortex. May play an important role in palate formation. Acts as a molecular node in a transcriptional network regulating skeletal development and osteoblast differentiation.

Tissue specificity

High expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.

Involvement in disease

Note=Chromosomal aberrations involving SATB2 are found in isolated cleft palate. Translocation t(2;7); translocation t(2;11).
Defects in SATB2 are a cause of cleft palate isolated (CPI) [MIM:119540]. A congenital fissure of the soft and/or hard palate, due to faulty fusion. Isolated cleft palate is not associated with cleft lips. Some patients may manifest other craniofacial dysmorphic features, mental retardation, and osteoporosis.
Note=A chromosomal aberration involving SATB2 is found in a patient with classical features of Toriello-Carey syndrome. Translocation t(2;14)(q33;q22).

Sequence similarities

Belongs to the CUT homeobox family.
Contains 2 CUT DNA-binding domains.
Contains 1 homeobox DNA-binding domain.

Post-translational
modifications

Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity.

Cellular localization

Nucleus matrix.

Target information above from: UniProt accessionQ9UPW6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DNA binding protein SATB2 antibody
  • DNA-binding protein SATB2 antibody
  • SATB2 antibody
  • SATB2_HUMAN antibody
  • Special AT rich sequence binding protein 2 antibody
  • Special AT-rich sequence-binding protein 2 antibody
see all

Database links

see all

Anti-SATB2 antibody [SATBA4B10] images:

  Western blot - SATB2 antibody [SATBA4B10] (ab51502)

Western blot - SATB2 antibody [SATBA4B10] (ab51502)

Anti-SATB2 antibody [SATBA4B10] (ab51502) at 1/100 dilution + NIH3T3 whole cell lysate at 25 µg

Secondary
Anti Mouse IgG antibody at 1/2500 dilution

Predicted band size : 81 kDa
Observed band size : 85 kDa (why is the actual band size different from the predicted?)


Same results obtained using HT-1080 whole cell lysate at 25μg.

  Immunoprecipitation - SATB2 antibody [SATBA4B10] (ab51502)

Immunoprecipitation - SATB2 antibody [SATBA4B10] (ab51502)

Immunoprecipitation using ab51502 at 1:100 dilution. Sample :NIH3T3 whole cell lysate 25µg. Secondary used at 1:2500.

  Immunocytochemistry/ Immunofluorescence - SATB2 antibody [SATBA4B10] (ab51502)

Immunocytochemistry/ Immunofluorescence - SATB2 antibody [SATBA4B10] (ab51502)

ab51502 staining SATB2 in HeLa cells by Immunocytochemistry 1:200.

  Immunocytochemistry/ Immunofluorescence - SATB2 antibody [SATBA4B10] (ab51502)

Immunocytochemistry/ Immunofluorescence - SATB2 antibody [SATBA4B10] (ab51502)

ab51502 staining cultured rat cortical neuron cells by ICC/IF.  Cells were PFA fixed, permeabilized in 0.1% triton and blocked with 10% BSA for 1 hour at 22°C.  The sample was then incubated with ab51502, diluted 1/25 for 2 hours at 22°C prior to incubation with a Cy3® conjugated donkey anti-mouse antibody, diluted 1/400.  In the image, red is SATB2 and green is F-actin

This image is courtesy of an Abreview submitted by Dr Ioana Carcea

See Abreview

  Immunohistochemistry (Frozen sections) - SATB2 antibody [SATBA4B10] (ab51502)

Immunohistochemistry (Frozen sections) - SATB2 antibody [SATBA4B10] (ab51502)

ab51502 staining SATB2 in mouse brain tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 22°C. The sample was incubated with primary antibody (1/500) at 4°C for 16 hours. An Alexa Fluor®488-conjugated Goat polyclonal to mouse IgG (1/1000) was used as secondary antibody.

This image is courtesy of an anonymous Abreview

See Abreview

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SATB2 antibody [SATBA4B10] (ab51502)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SATB2 antibody [SATBA4B10] (ab51502)

ab51502 staining SATB2 in human fetal cortex tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer pH 6.0. Samples were then blocked with 10% serum for 1 hour at 25°C followed by incubation with the primary antibody at a 1/100 dilution for 24 hours at 4°C. A Cy3®-conjugated horse anti-mouse polyclonal was used as secondary antibody at a 1/200 dilution. Counter-stained with DAPI (blue).

Image courtesy of an anonymous Abreview.

See Abreview

  Immunocytochemistry/ Immunofluorescence - SATB2 antibody [SATBA4B10] (ab51502)

Immunocytochemistry/ Immunofluorescence - SATB2 antibody [SATBA4B10] (ab51502)

ab51502 staining SATB2 (red) in a mouse cortex e17.5 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 3% Donkey serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/10) for 90 minutes at 22°C. An Alexa Fluor®555-conjugated Donkey anti-mouse polyclonal (1/200) was used as the secondary antibody.
Green: CTIP2 (ab18465) staining
Blue: TBR1 (ab31940) staining

This image is courtesy of an anonymous Abreview

See Abreview

References for Anti-SATB2 antibody [SATBA4B10] (ab51502)

This product has been referenced in:

  • Asanoma Ket al. SATB Homeobox Proteins Regulate Trophoblast Stem Cell Renewal and Differentiation. J Biol Chem 287:2257-68 (2012). WB, ICC/IF; Rat.Read more (PubMed: 22123820) »
  • Wang Het al. Gli3 is required for maintenance and fate specification of cortical progenitors. J Neurosci 31:6440-8 (2011).Read more (PubMed: 21525285) »

See all 9 publications for this product

Publishing research using ab51502? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"