Overview

  • Product nameAnti-SAV1 antibody
    See all SAV1 primary antibodies
  • Description
    Rabbit polyclonal to SAV1
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Monkey, Macaque Monkey, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human SAV1.

  • Positive control
    • This antibody gave a positive signal in both adult and fetal Heart tissue lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab105105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 45 kDa).

Target

  • FunctionRegulator of STK3/MST2 and STK4/MST1 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. SAV1 is required for STK3/MST2 and STK4/MST1 activation and promotes cell-cycle exit and terminal differentiation in developing epithelial tissues. Plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosomes, and its ability to phosphorylate CROCC and CEP250. In conjunction with STK3/MST2, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation.
  • Tissue specificityUbiquitously expressed in adult tissues with highest expression in the pancreas, aorta and interventricular septum and lowest expression in skeletal muscle. Expression was higher in fetal than in the adult heart. Expressed in various cell lines.
  • Sequence similaritiesContains 1 SARAH domain.
    Contains 2 WW domains.
  • Post-translational
    modifications
    Phosphorylated by STK3/MST2 and STK4/MST1. Phosphorylation is not required for SAV1 stability and may increase the number of protein binding sites on the scaffold molecule.
  • Cellular localizationNucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1700040G09Rik antibody
    • 45 kDa WW domain protein antibody
    • hWW 45 antibody
    • hWW45 antibody
    • Protein salvador homolog 1 antibody
    • salvador family WW domain containing protein 1 antibody
    • salvador homolog 1 (Drosophila) antibody
    • Salvador homolog 1 antibody
    • Salvador, Drosophila, homolog of antibody
    • SAV 1 antibody
    • SAV antibody
    • SAV1 antibody
    • SAV1_HUMAN antibody
    • WW 45 antibody
    • WW domain containing antibody
    • WW domain-containing adaptor 45 antibody
    • WW domain-containing protein, 45-KD antibody
    • WW45 antibody
    • WW45 protein antibody
    • WWP 4 antibody
    • WWP4 antibody
    see all

Anti-SAV1 antibody images

  • All lanes : Anti-SAV1 antibody (ab105105) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Heart (Human) Whole Cell Lysate - fetal normal tissue

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 51 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 90 seconds
  • ICC/IF image of ab105105 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105105, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) Hek293 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HepG2 and MCF7 cells at 1µg/ml.

References for Anti-SAV1 antibody (ab105105)

ab105105 has not yet been referenced specifically in any publications.

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