Overview

  • Product nameSB 202190
  • Description
    Highly selective, potent, cell-permeable p38 MAPK inhibitor
  • Biological descriptionHighly selective, potent and cell-permeable p38 MAPK inhibitor (IC50 values are 50 and 100 nM at SAPK2a/p38 and SAPK2b/p38β2, respectively). Selective over a wide range of other kinases. Apoptosis and autophagy inducer.
  • Purity> 99%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120638 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    SB 202190 images

    • ab18528 staining Lamp2a in CaCO2 cells treated with SB 202190 (ab120638), by ICC/IF. Increase of Lamp2a expression correlates with increased concentration of SB 202190, as described in literature.
      The cells were incubated at 37°C for 3 hours in media containing different concentrations of ab120638 (SB 202190) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab18528 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for SB 202190 (ab120638)

    This product has been referenced in:
    • Menon MB  et al. SB202190-induced cell type-specific vacuole formation and defective autophagy do not depend on p38 MAP kinase inhibition. PLoS One 6:e23054 (2011). Read more (PubMed: 21853067) »
    • Davies SP  et al. Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem J 351:95-105 (2000). Read more (PubMed: 10998351) »
    • Karahashi H  et al. A selective inhibitor of p38 MAP kinase, SB202190, induced apoptotic cell death of a lipopolysaccharide-treated macrophage-like cell line, J774.1. Biochim Biophys Acta 1502:207-23 (2000). Read more (PubMed: 11040446) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"