Overview

  • Product name
    Anti-Sbf1 antibody [EPR12567]
    See all Sbf1 primary antibodies
  • Description
    Rabbit Monoclonal [EPR12567] to Sbf1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Sbf1 aa 1750-1850. The exact sequence is proprietary.
    Database link: O95248

  • Positive control
    • Human fetal brain lysate; Jurkat and Ramos cell lysates; Human brain and nuscle tissues; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12567
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 208 kDa (predicted molecular weight: 208 kDa).
IHC-P 1/250 - 1/500.
Flow Cyt 1/210.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Relevance
    Probable pseudophosphatase. Lacks several amino acids in the catalytic pocket which renders it catalytically inactive as a phosphatase. The pocket is however sufficiently preserved to bind phosphorylated substrates, and maybe protect them from phosphatases. Inhibits myoblast differentiation in vitro and induces oncogenic transformation in fibroblasts. May function as a guanine nucleotide exchange factor (GEF) activating RAB28. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form.
  • Cellular localization
    Nuclear
  • Database links
  • Alternative names
    • C22:RP4-579N16.2 antibody
    • CMT4B3 antibody
    • DENN/MADD domain containing 7A antibody
    • DENND7A antibody
    • MTMR5 antibody
    • Myotubularin related 5 antibody
    • Myotubularin-related protein 5 antibody
    • SET-binding factor 1 antibody
    see all

Images

  • Anti-Sbf1 antibody [EPR12567] (ab181106) at 1/5000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 208 kDa
    Observed band size: 208 kDa

  • All lanes : Anti-Sbf1 antibody [EPR12567] (ab181106) at 1/5000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Ramos cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 208 kDa
    Observed band size: 208 kDa

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Sbf1 with ab181106 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human muscle tissue labeling Sbf1 with ab181106 at 1/500 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling Sbf1 with ab181106 at 1/210 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

References

This product has been referenced in:

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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