Overview

  • Product name
    Anti-SBF2 antibody
  • Description
    Rabbit polyclonal to SBF2
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A recombinant fragment, corresponding to amino acids 1654-1728 of Human SBF2 (UniProt: Q86WG5).

  • Positive control
    • Human cervix tissue. RT 4 and U 251 MG cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab151110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500. Predicted molecular weight: 208 kDa.
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Relevance
    Defects in SBF2 are the cause of Charcot-Marie-Tooth disease type 4B2 (CMT4B2). CMT4B2 is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4. CMT4B2 is characterized by abnormal folding of myelin sheaths. CMT4B2 is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4. CMT4B2 is characterized by abnormal folding of myelin sheaths.
  • Cellular localization
    Cell Membrane and Cytoplasmic. Peripheral membrane protein.
  • Database links
  • Alternative names
    • CMT4B2 antibody
    • KIAA1766 antibody
    • MTMR13 antibody
    • Myotubularin-related protein 13 antibody
    • SET-binding factor 2 antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab151110 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemical analysis of paraffin-embedded Human cervix tissue labeling SBF2 with ab151110 at 1/200 dilution.
  • All lanes : Anti-SBF2 antibody (ab151110) at 1/250 dilution

    Lane 1 : RT 4 cell lysate
    Lane 2 : U 251 MG cell lysate
    Lane 3 : Human plasma lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human tonsil tissue lysate


    Predicted band size : 208 kDa

References

ab151110 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab151110.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up