The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 - 4 µg/ml.
Streptavidin Binding Peptide (SBP) tag is very versatile and can be inserted into expression vectors for easy detection of recombinant proteins and purification by use of affinity columns. The
full sequence for SBP-tag is 38 amino acids long and exhibits a nanomolar scale binding affinity for streptavidin.