Anti-SC35 antibody (ab116103)

Overview

  • Product nameAnti-SC35 antibody
    See all SC35 primary antibodies
  • Description
    Rabbit polyclonal to SC35
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Guinea pig, Cow, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 170-219 (KSKSSSVSRS RSRSRSRSRS RSPPPVSKRE SKSRSRSKSP PKSPEEEGAV) of Human SC35 (NP_003007).

  • Positive control
    • HeLa cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab116103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionNecessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway.
  • Sequence similaritiesBelongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Extensively phosphorylated on serine residues in the RS domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 35 kDa antibody
    • arginine/serine-rich 2 antibody
    • PR264 antibody
    • Protein PR264 antibody
    • SC 35 antibody
    • SC-35 antibody
    • SC35 antibody
    • Serine/arginine-rich splicing factor 2 antibody
    • SFRS 2 antibody
    • SFRS2 antibody
    • SFRS2A antibody
    • Splicing component 35 kDa antibody
    • Splicing component antibody
    • Splicing factor antibody
    • Splicing factor arginine/serine rich 2 antibody
    • Splicing factor SC35 antibody
    • Splicing speckle antibody
    • Splicing speckles antibody
    • SR splicing factor 2 antibody
    • SRp30b antibody
    • SRSF2 antibody
    • SRSF2_HUMAN antibody
    see all

Anti-SC35 antibody images

  • Anti-SC35 antibody (ab116103) at 1 µg/ml + HeLa cell lysate at 10 µg

    Predicted band size : 25 kDa

References for Anti-SC35 antibody (ab116103)

ab116103 has not yet been referenced specifically in any publications.

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Thank you for submitting an Abreview of ab116103. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in a untested species and application (mouse, ICC/IF), we would like t...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH/3T3 mouse embryonic fibroblast)
Specification NIH/3T3 mouse embryonic fibroblast
Fixative Formaldehyde
Permeabilization Yes - PBS with 0.5% Triton-X100
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

Submitted Jun 01 2012

DISCOUNT CODES:

ab115184: x


ab116103: y


Ablaufdatum jeweils: dd mm 2012

Es freut mich zu hören, dass Sie unser Angebot unsere Antikörper der IF zu testen, annehmen möchten. Wie besprochen...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"