Anti-SC35 antibody (ab116103)

Overview

  • Product name
  • Description
    Rabbit polyclonal to SC35
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Guinea pig, Cow, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 170-219 (KSKSSSVSRS RSRSRSRSRS RSPPPVSKRE SKSRSRSKSP PKSPEEEGAV) of Human SC35 (NP_003007).

  • Positive control
    • HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab116103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 25 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway.
  • Sequence similarities
    Belongs to the splicing factor SR family.
    Contains 1 RRM (RNA recognition motif) domain.
  • Post-translational
    modifications
    Extensively phosphorylated on serine residues in the RS domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 35 kDa antibody
    • arginine/serine-rich 2 antibody
    • PR264 antibody
    • Protein PR264 antibody
    • SC 35 antibody
    • SC-35 antibody
    • SC35 antibody
    • Serine/arginine-rich splicing factor 2 antibody
    • SFRS 2 antibody
    • SFRS2 antibody
    • SFRS2A antibody
    • Splicing component 35 kDa antibody
    • Splicing component antibody
    • Splicing factor antibody
    • Splicing factor arginine/serine rich 2 antibody
    • Splicing factor SC35 antibody
    • Splicing speckle antibody
    • Splicing speckles antibody
    • SR splicing factor 2 antibody
    • SRp30b antibody
    • SRSF2 antibody
    • SRSF2_HUMAN antibody
    see all

Images

  • Anti-SC35 antibody (ab116103) at 1 µg/ml + HeLa cell lysate at 10 µg

    Predicted band size : 25 kDa

References

ab116103 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Thank you for submitting an Abreview of ab116103. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in a untested species and application (mouse, ICC/IF), we would like t...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH/3T3 mouse embryonic fibroblast)
Specification
NIH/3T3 mouse embryonic fibroblast
Fixative
Formaldehyde
Permeabilization
Yes - PBS with 0.5% Triton-X100
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Jun 01 2012

DISCOUNT CODES:

ab115184: x


ab116103: y


Ablaufdatum jeweils: dd mm 2012

Es freut mich zu hören, dass Sie unser Angebot unsere Antikörper der IF zu testen, annehmen möchten. Wie besprochen...

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