Anti-Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)

Overview

  • Product nameAnti-Sca1 / Ly6A/E antibody [D7] (FITC)
    See all Sca1 / Ly6A/E primary antibodies
  • Description
    Rat monoclonal [D7] to Sca1 / Ly6A/E (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityThis antibody is specific for Ly6A.2 and Ly6E.1, allelic members of the Ly6 multigene family.
  • Tested applicationsICC/IF, Flow Cyt, IP, WB, IHC-Fr, Cellular activation, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    IL2 dependent T cell line CTL-L (Mouse).

Properties

Applications

Our Abpromise guarantee covers the use of ab25031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. Ab18446-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.
IHC-Fr Use at an assay dependent concentration.
Cellular activation Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 21858462

Target

  • FunctionT-cell activation.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesContains 1 UPAR/Ly6 domain.
  • Post-translational
    modifications
    O-glycosylated. Not N-glycosylated.
    Not phosphorylated.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ly-6A.2/Ly-6E. antibody
    • Ly-6A.2/Ly-6E.1 antibody
    • Ly6a antibody
    • Ly6A.2 antibody
    • Ly6A/E antibody
    • LY6A_MOUSE antibody
    • Ly6al antibody
    • Ly6E.1 antibody
    • Lymphocyte antigen 6 complex locus A antibody
    • Lymphocyte antigen 6 complex locus E antibody
    • Lymphocyte antigen 6A-2/6E-1 antibody
    • Lymphocyte antigen Ly 6A.2/Ly 6E.1 antibody
    • Retinoic acid induced gene E antibody
    • RIGE antibody
    • SCA-1 antibody
    • Sca1 antibody
    • Sca2 antibody
    • Stem cell antigen 1 antibody
    • Stem cell antigen 2 antibody
    • T cell activating protein antibody
    • T-cell-activating protein antibody
    • TAP antibody
    • Thymic shared antigen 1 antibody
    • TSA1 antibody
    see all

Anti-Sca1 / Ly6A/E antibody [D7] (FITC) images

  • BALB/c spleen cells were activated by incubation for 48 hours with concanavalin A. After harvesting, the cells were stained with Rat anti-mouse Ly6A/E-FITC (less than or equal to 0.5 ug/106 cells) and activated cells were gated and analyzed on a FACScan flow cytometer.
  • ICC/IF image of ab25031 stained MEF cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25031, 5µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)

This product has been referenced in:
  • Wang H  et al. Over-expression of PDGFR-ß promotes PDGF-induced proliferation, migration, and angiogenesis of EPCs through PI3K/Akt signaling pathway. PLoS One 7:e30503 (2012). Flow Cyt ; Mouse . Read more (PubMed: 22355314) »
  • Mahdipour E  et al. Hoxa3 promotes the differentiation of hematopoietic progenitor cells into proangiogenic Gr-1+CD11b+ myeloid cells. Blood 117:815-26 (2011). Flow Cyt ; Mouse . Read more (PubMed: 20974673) »

See all 6 Publications for this product

Product Wall

Application Flow Cytometry
Fixation None, cells were used fresh
Permeabilization No
Sample Mouse Cell (Cells from bone marrow)
Specification Cells from bone marrow
Gating Strategy Alive cells (DAPI-), blood cells exluded
Preparation Cell harvesting/tissue preparation method: Bone marrow from Femur was extracted with PBS-EDTA 3mM, 2 hours before flow cytometry analysis
Sample buffer: PBS-EDTA 3mM
Username

Dr. Francois Daubeuf

Verified customer

Submitted Feb 24 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Bone marrow)
Specification Bone marrow
Fixative Formaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 09 2012

Thank you for contacting us. We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable. We encour...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"