Overview

  • Product nameAnti-Sca1 / Ly6A/E antibody [D7]
    See all Sca1 / Ly6A/E primary antibodies
  • Description
    Rat monoclonal [D7] to Sca1 / Ly6A/E
  • Specificityab25195 is specific for Ly-6A.2 and Ly-6E.1, allelic members of the Ly-6 multigene family.
  • Tested applicationsSuitable for: Flow Cyt, IP, WB, IHC-Fr, Celluar Activation, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    IL-2-dependent mouse T-cell line CTL-L

Properties

Applications

Our Abpromise guarantee covers the use of ab25195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 18 kDa.
IHC-Fr Use at an assay dependent concentration.
Celluar Activation Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • FunctionT-cell activation.
  • Tissue specificityWidely expressed.
  • Sequence similaritiesContains 1 UPAR/Ly6 domain.
  • Post-translational
    modifications
    O-glycosylated. Not N-glycosylated.
    Not phosphorylated.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ly-6A.2/Ly-6E. antibody
    • Ly-6A.2/Ly-6E.1 antibody
    • Ly6a antibody
    • LY6A_MOUSE antibody
    • Ly6al antibody
    • Lymphocyte antigen 6A-2/6E-1 antibody
    • Lymphocyte antigen Ly 6A.2/Ly 6E.1 antibody
    • SCA-1 antibody
    • Stem cell antigen 1 antibody
    • T cell activating protein antibody
    • T-cell-activating protein antibody
    • TAP antibody
    see all

Anti-Sca1 / Ly6A/E antibody [D7] images

  • Flow Cytometry analysis of Con-A-stimulated BALB/c mouse splenocytes labeling Sca1 / Ly6A/E with ab25195 at 1 μg/106 cells (purple). A Mouse Anti-Rat IgG2a-AF647 was used as the secondary antibody. Grey - Isotype Control, Rat IgG2a-UNLB, followed by Mouse Anti-Rat IgG2a-AF647.

References for Anti-Sca1 / Ly6A/E antibody [D7] (ab25195)

This product has been referenced in:
  • Neirinckx V  et al. Adult bone marrow mesenchymal and neural crest stem cells are chemoattractive and accelerate motor recovery in a mouse model of spinal cord injury. Stem Cell Res Ther 6:211 (2015). WB ; Mouse . Read more (PubMed: 26530515) »
  • Malliaras K  et al. Stimulation of endogenous cardioblasts by exogenous cell therapy after myocardial infarction. EMBO Mol Med 6:760-77 (2014). ICC/IF ; Mouse . Read more (PubMed: 24797668) »

See all 7 Publications for this product

Product Wall

I agree that the staining seems incorrect. In two of the images, a subset of cells at the edges of the sections are staining, and in the third, the staining is of something other than cells, maybe extracellular matrix.

The following article ...

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