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IL-2-dependent mouse T-cell line CTL-L
Our Abpromise guarantee covers the use of ab25195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 18 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
|Cellular Activation||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
Flow Cytometry analysis of Con-A-stimulated BALB/c mouse splenocytes labeling Sca1 / Ly6A/E with ab25195 at 1 μg/106 cells (purple). A Mouse Anti-Rat IgG2a-AF647 was used as the secondary antibody. Grey - Isotype Control, Rat IgG2a-UNLB, followed by Mouse Anti-Rat IgG2a-AF647.