Anti-Sca1 / Ly6A/E antibody [D7] (Allophycocyanin) (ab25323)

Overview

  • Product nameAnti-Sca1 / Ly6A/E antibody [D7] (Allophycocyanin)
    See all Sca1 / Ly6A/E primary antibodies
  • Description
    Rat monoclonal [D7] to Sca1 / Ly6A/E (Allophycocyanin)
  • ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
  • Specificityab25323 is specific for Ly-6A.2 and Ly-6E.1, allelic members of the Ly-6 multigene family.
  • Tested applicationsSuitable for: Flow Cyt, WB, IP, Celluar Activation, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation (Mouse)

Properties

Applications

Our Abpromise guarantee covers the use of ab25323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
WB
IP
Celluar Activation
IHC-Fr
  • Application notesCellular activation: Use at an assay dependent dilution.
    Flow Cyt: Use 0.2µg for 106 cells.
    IHC-Fr: Use at an assay dependent dilution.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 18 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionT-cell activation.
    • Tissue specificityWidely expressed.
    • Sequence similaritiesContains 1 UPAR/Ly6 domain.
    • Post-translational
      modifications
      O-glycosylated. Not N-glycosylated.
      Not phosphorylated.
    • Cellular localizationCell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ly-6A.2/Ly-6E. antibody
      • Ly-6A.2/Ly-6E.1 antibody
      • Ly6a antibody
      • LY6A_MOUSE antibody
      • Ly6al antibody
      • Lymphocyte antigen 6A-2/6E-1 antibody
      • Lymphocyte antigen Ly 6A.2/Ly 6E.1 antibody
      • SCA-1 antibody
      • Stem cell antigen 1 antibody
      • T cell activating protein antibody
      • T-cell-activating protein antibody
      • TAP antibody
      see all

    References for Anti-Sca1 / Ly6A/E antibody [D7] (Allophycocyanin) (ab25323)

    This product has been referenced in:
    • Ivanov V  et al. Regulation of nuclear factor-kappa B and activator protein-1 activities after stimulation of T cells via glycosylphosphatidylinositol-anchored Ly-6A/E. J Immunol 153:2394-406 (1994). Read more (PubMed: 7915738) »
    • Flood PM  et al. Inhibition of Ly-6A antigen expression prevents T cell activation. J Exp Med 172:115-20 (1990). Read more (PubMed: 2141625) »

    See all 6 Publications for this product

    Product Wall

    Thank you for your enquiry.

    I believe we will be able to add an appropriate rat IgG2a, Allophycocyanin conjugatedisotype control to our catalog. I will let you know as soon as it is available for sale.

    Please let me know if you have...

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    Thank you for your call earlier this week, and for your patience while I have been in touch with the lab. For both of these antibodies, you can follow the direct flow cytometry protocol on our website- http://www.abcam.com/index.html?pageconfig=...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"