Anti-Sca1 / Ly6A/E antibody [D7] (FITC) (ab25031)

Overview

  • Product name
    Anti-Sca1 / Ly6A/E antibody [D7] (FITC)
    See all Sca1 / Ly6A/E primary antibodies
  • Description
    Rat monoclonal [D7] to Sca1 / Ly6A/E (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    This antibody is specific for Ly6A.2 and Ly6E.1, allelic members of the Ly6 multigene family.
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IP, WB, IHC-Fr, Cellular Activation, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    IL2 dependent T cell line CTL-L (Mouse).

Properties

Applications

Our Abpromise guarantee covers the use of ab25031 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab18446-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.
IHC-Fr Use at an assay dependent concentration.
Cellular Activation Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 21858462

Target

  • Function
    T-cell activation.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Contains 1 UPAR/Ly6 domain.
  • Post-translational
    modifications
    O-glycosylated. Not N-glycosylated.
    Not phosphorylated.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ly-6A.2/Ly-6E. antibody
    • Ly-6A.2/Ly-6E.1 antibody
    • Ly6a antibody
    • LY6A_MOUSE antibody
    • Ly6al antibody
    • Lymphocyte antigen 6A-2/6E-1 antibody
    • Lymphocyte antigen Ly 6A.2/Ly 6E.1 antibody
    • SCA-1 antibody
    • Stem cell antigen 1 antibody
    • T cell activating protein antibody
    • T-cell-activating protein antibody
    • TAP antibody
    see all

Images

  • ICC/IF image of ab25031 stained MEF cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25031, 5µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • BALB/c spleen cells were activated by incubation for 48 hours with concanavalin A. After harvesting, the cells were stained with Rat anti-mouse Ly6A/E-FITC (less than or equal to 0.5 ug/106 cells) and activated cells were gated and analyzed on a FACScan flow cytometer.
  • Flow Cytometry analysis of Con-A stimulated C57BL/6 mouse splenocytes labeling Ly-6A with ab25031 at 3 μg/106 cells dilution (purple). Grey - Isotype Control, Rat IgG2a-FITC.

References

This product has been referenced in:
  • Zhang L  et al. Transcription factor E2-2 inhibits the proliferation of endothelial progenitor cells by suppressing autophagy. Int J Mol Med N/A:N/A (2016). Flow Cyt ; Mouse . Read more (PubMed: 26986900) »
  • Sui B  et al. Allogeneic Mesenchymal Stem Cell Therapy Promotes Osteoblastogenesis and Prevents Glucocorticoid-Induced Osteoporosis. Stem Cells Transl Med 5:1238-46 (2016). Read more (PubMed: 27365487) »

See all 12 Publications for this product

Customer reviews and Q&As

Application
Flow Cytometry
Fixation
None, cells were used fresh
Permeabilization
No
Sample
Mouse Cell (Cells from bone marrow)
Specification
Cells from bone marrow
Gating Strategy
Alive cells (DAPI-), blood cells exluded
Preparation
Cell harvesting/tissue preparation method: Bone marrow from Femur was extracted with PBS-EDTA 3mM, 2 hours before flow cytometry analysis
Sample buffer: PBS-EDTA 3mM
Username

Dr. Francois Daubeuf

Verified customer

Submitted Feb 24 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Bone marrow)
Specification
Bone marrow
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 09 2012

Thank you for contacting us. We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field and do not want to recommend a product that may be unsuitable. We encour...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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