IL2 dependent T cell line CTL-L (Mouse).
Our Abpromise guarantee covers the use of ab25031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|Flow Cyt||Use 1µg for 106 cells. ab18446-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 14 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
|Cellular Activation||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. PubMed: 21858462|
Flow Cytometry analysis of Con-A stimulated C57BL/6 mouse splenocytes labeling Ly-6A with ab25031 at 3 μg/106 cells dilution (purple). Grey - Isotype Control, Rat IgG2a-FITC.