ICC/IF image of ab25031 stained MEF cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25031, 5µg/ml) overnight at +4°C. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
BALB/c spleen cells were activated by incubation for 48 hours with concanavalin A. After harvesting, the cells were stained with Rat anti-mouse Ly6A/E-FITC (less than or equal to 0.5 ug/106 cells) and activated cells were gated and analyzed on a FACScan flow cytometer.
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Sui B et al. Allogeneic Mesenchymal Stem Cell Therapy Promotes Osteoblastogenesis and Prevents Glucocorticoid-Induced Osteoporosis. Stem Cells Transl Med5:1238-46 (2016).
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