Anti-Sca1 / Ly6A/E antibody [D7] - Low endotoxin, Azide free (ab174602)

Overview

  • Product name
    Anti-Sca1 / Ly6A/E antibody [D7] - Low endotoxin, Azide free
    See all Sca1 / Ly6A/E primary antibodies
  • Description
    Rat monoclonal [D7] to Sca1 / Ly6A/E - Low endotoxin, Azide free
  • Tested applications
    Suitable for: Flow Cyt, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control
    • Mouse splenocyte and bone marrow cell suspensions.

Properties

Applications

Our Abpromise guarantee covers the use of ab174602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.5µg for 105-8 cells.

(Final test volume = 100 µL).

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Functional Studies Use at an assay dependent concentration.

Target

  • Function
    T-cell activation.
  • Tissue specificity
    Widely expressed.
  • Sequence similarities
    Contains 1 UPAR/Ly6 domain.
  • Post-translational
    modifications
    O-glycosylated. Not N-glycosylated.
    Not phosphorylated.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Ly-6A.2/Ly-6E. antibody
    • Ly-6A.2/Ly-6E.1 antibody
    • Ly6a antibody
    • LY6A_MOUSE antibody
    • Ly6al antibody
    • Lymphocyte antigen 6A-2/6E-1 antibody
    • Lymphocyte antigen Ly 6A.2/Ly 6E.1 antibody
    • SCA-1 antibody
    • Stem cell antigen 1 antibody
    • T cell activating protein antibody
    • T-cell-activating protein antibody
    • TAP antibody
    see all

Images

  • Flow cytometry analysis of 3 day ConA activated C57B1/6 Mouse splenocytes labeling Sca1 / Ly6A/E with ab174602 (filled histogram) at 0.5 µg or purified Rat IgG2a Isotype Control (open histogram) at 0.5 µg, followed by an anti-Rat IgG FITC. Total viable cells were used for analysis.

References

ab174602 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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