Anti-Sca1 / Ly6A/E antibody [D7] (PE/Cy7 ®) (ab93537)

Overview

  • Product name
    Anti-Sca1 / Ly6A/E antibody [D7] (PE/Cy7 ®)
    See all Sca1 / Ly6A/E primary antibodies
  • Description
    Rat monoclonal [D7] to Sca1 / Ly6A/E (PE/Cy7 ®)
  • Conjugation
    PE/Cy7 ®. Ex: 496nm, Em: 774nm
  • Specificity
    ab93537 reacts with both Ly6E.1 and Ly6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    IL2 dependent T cell line CTL-L (Mouse).

  • Positive control
    • C57Bl/6 splenocytes.
  • General notes
    This Cy7 tandem dye is sensitive to photo-induced oxidation. Protect this reagent from light during storage, handling & experimental procedures.

Properties

Applications

Our Abpromise guarantee covers the use of ab93537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use at a concentration of 0.06 µg/test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      T-cell activation.
    • Tissue specificity
      Widely expressed.
    • Sequence similarities
      Contains 1 UPAR/Ly6 domain.
    • Post-translational
      modifications
      O-glycosylated. Not N-glycosylated.
      Not phosphorylated.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ly-6A.2/Ly-6E. antibody
      • Ly-6A.2/Ly-6E.1 antibody
      • Ly6a antibody
      • LY6A_MOUSE antibody
      • Ly6al antibody
      • Lymphocyte antigen 6A-2/6E-1 antibody
      • Lymphocyte antigen Ly 6A.2/Ly 6E.1 antibody
      • SCA-1 antibody
      • Stem cell antigen 1 antibody
      • T cell activating protein antibody
      • T-cell-activating protein antibody
      • TAP antibody
      see all

    Images

    • ab93537 at 0.06µg dilution staining Sca1 / Ly6A/E in C57Bl/6 splenocytes by Flow Cytometry. Cells in the lymphocyte gate were used for analysis.

    References

    ab93537 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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