Anti-Sca1 / Ly6A/E antibody [E13 161-7] (ab51317)

Overview

  • Product name
    Anti-Sca1 / Ly6A/E antibody [E13 161-7]
    See all Sca1 / Ly6A/E primary antibodies
  • Description
    Rat monoclonal [E13 161-7] to Sca1 / Ly6A/E
  • Specificity
    This antibody reacts with an antigen expressed on mouse hematopoietic stem cells.
  • Tested applications
    Suitable for: ICC/IFmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation of Mouse Sca1/ Ly6A/E.

  • Positive control
    • ICC/IF: TH2 cell line.
  • General notes

    This antibody clone is manufactured by Abcam.

Properties

Applications

Our Abpromise guarantee covers the use of ab51317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      T-cell activation.
    • Tissue specificity
      Widely expressed.
    • Sequence similarities
      Contains 1 UPAR/Ly6 domain.
    • Post-translational
      modifications
      O-glycosylated. Not N-glycosylated.
      Not phosphorylated.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ly-6A.2/Ly-6E. antibody
      • Ly-6A.2/Ly-6E.1 antibody
      • Ly6a antibody
      • LY6A_MOUSE antibody
      • Ly6al antibody
      • Lymphocyte antigen 6A-2/6E-1 antibody
      • Lymphocyte antigen Ly 6A.2/Ly 6E.1 antibody
      • SCA-1 antibody
      • Stem cell antigen 1 antibody
      • T cell activating protein antibody
      • T-cell-activating protein antibody
      • TAP antibody
      see all

    Images

    • ab51317 stained TH2 cells. The cells were 4% formaldehyde fixed for 4 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51317 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150165) used at a 1/1000 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • Negative control cell line:

      ab51317 showing no staining in NIH-3T3 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51317 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150165) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    References

    This product has been referenced in:
    • James AW  et al. NELL-1 induces Sca-1+ mesenchymal progenitor cell expansion in models of bone maintenance and repair. JCI Insight 2:N/A (2017). IHC-P ; Mouse . Read more (PubMed: 28614787) »
    • Cuttano R  et al. KLF4 is a key determinant in the development and progression of cerebral cavernous malformations. EMBO Mol Med 8:6-24 (2016). Read more (PubMed: 26612856) »

    See all 16 Publications for this product

    Customer reviews and Q&As

    I agree that the staining seems incorrect. In two of the images, a subset of cells at the edges of the sections are staining, and in the third, the staining is of something other than cells, maybe extracellular matrix.

    The following article ...

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    How do the stains with 1/500 compare to the other dilutions? Is the background intensity the same? Do you see anything that appears to be a specific stain?

    One reagent that is sometimes added to blocking buffers is glycine, to block free alde...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Bone marrow)
    Specification
    Bone marrow
    Fixative
    Formaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 12 2012

    Thank you for you reply.

    Your credit note IDs are ***** & *********.

    I am sorry that these antibodies did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you for all of the...

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    Thank you for contacting Abcam.

    I am sorry about all of the problems you have been having with these antibodies.

    It would greatly be appreciated if you could send me some of the images that you are getting using these antibodies and...

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    Thank you for contacting us. Because we carry over 80,000 products, it isn't feasible for us to keep small sample sizes of our products.

    We are happy to reassure our customers that all of our products - including the Sca1/Ly6A/E antibody ab5...

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    Vielen Dank für diese zusätzlichen Informationen.
    Ich würde Sie bitten die folgenden drei Tipps auszuprobieren und möchte Ihnen nochmals versichern, dass wenn die Protokolltipps das Resultat nicht verbessern, die Abpromise Garantie greift und ich ...

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    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Skin)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 08 2010

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
    Permeabilization
    No
    Specification
    Kidney
    Blocking step
    5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Formaldehyde
    Username

    Mr. Antibody Solutions

    Verified customer

    Submitted Apr 08 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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