Anti-Sca1 / Ly6A/E antibody [E13 161-7] (ab51317)

Overview

  • Product name
    Anti-Sca1 / Ly6A/E antibody [E13 161-7]
    See all Sca1 / Ly6A/E primary antibodies
  • Description
    Rat monoclonal [E13 161-7] to Sca1 / Ly6A/E
  • Host species
    Rat
  • Specificity
    This antibody reacts with an antigen expressed on mouse hematopoietic stem cells.
  • Tested applications
    Suitable for: ICC/IFmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation of Mouse Sca1/ Ly6A/E.

  • Positive control
    • ICC/IF: TH2 cell line.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab51317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      T-cell activation.
    • Tissue specificity
      Widely expressed.
    • Sequence similarities
      Contains 1 UPAR/Ly6 domain.
    • Post-translational
      modifications
      O-glycosylated. Not N-glycosylated.
      Not phosphorylated.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ly-6A.2/Ly-6E. antibody
      • Ly-6A.2/Ly-6E.1 antibody
      • Ly6a antibody
      • LY6A_MOUSE antibody
      • Ly6al antibody
      • Lymphocyte antigen 6A-2/6E-1 antibody
      • Lymphocyte antigen Ly 6A.2/Ly 6E.1 antibody
      • SCA-1 antibody
      • Stem cell antigen 1 antibody
      • T cell activating protein antibody
      • T-cell-activating protein antibody
      • TAP antibody
      see all

    Images

    • ab51317 stained TH2 cells. The cells were 4% formaldehyde fixed for 4 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51317 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150165) used at a 1/1000 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    • Negative control cell line:

      ab51317 showing no staining in NIH-3T3 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51317 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150165) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

    References

    This product has been referenced in:
    • James AW  et al. NELL-1 induces Sca-1+ mesenchymal progenitor cell expansion in models of bone maintenance and repair. JCI Insight 2:N/A (2017). IHC-P ; Mouse . Read more (PubMed: 28614787) »
    • Wang L  et al. Aberrant Transforming Growth Factor-ß Activation Recruits Mesenchymal Stem Cells During Prostatic Hyperplasia. Stem Cells Transl Med 6:394-404 (2017). IHC-P ; Mouse . Read more (PubMed: 28191756) »

    See all 19 Publications for this product

    Customer reviews and Q&As

    Filter by Application

    Filter by Species

    Filter by Ratings

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Bone marrow)
    Specification
    Bone marrow
    Fixative
    Formaldehyde
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 12 2012

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Skin)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    Skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 08 2010

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Kidney)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Retrieval Solution
    Permeabilization
    No
    Specification
    Kidney
    Blocking step
    5 minutes of peroxidase block then 10 minutes of protein block. These are ready-to-use reagents purchased from Dako as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Formaldehyde
    Username

    Mr. Antibody Solutions

    Verified customer

    Submitted Apr 08 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up