Recombinant
RabMAb

Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211)

Overview

  • Product name
    Anti-Sca1 / Ly6A/E antibody [EPR3355]
    See all Sca1 / Ly6A/E primary antibodies
  • Description
    Rabbit monoclonal [EPR3355] to Sca1 / Ly6A/E
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to residues in Mouse Sca1/Ly6A/E.

  • Positive control
    • Mouse kidney cell lysate; Mouse kidney tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Human, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).
IHC-P 1/50 - 1/100. Antigen retrieval is recommended.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      T-cell activation.
    • Tissue specificity
      Widely expressed.
    • Sequence similarities
      Contains 1 UPAR/Ly6 domain.
    • Post-translational
      modifications
      O-glycosylated. Not N-glycosylated.
      Not phosphorylated.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ly-6A.2/Ly-6E. antibody
      • Ly-6A.2/Ly-6E.1 antibody
      • Ly6a antibody
      • LY6A_MOUSE antibody
      • Ly6al antibody
      • Lymphocyte antigen 6A-2/6E-1 antibody
      • Lymphocyte antigen Ly 6A.2/Ly 6E.1 antibody
      • SCA-1 antibody
      • Stem cell antigen 1 antibody
      • T cell activating protein antibody
      • T-cell-activating protein antibody
      • TAP antibody
      see all

    Images

    • Anti-Sca1 / Ly6A/E antibody [EPR3355] (ab109211) at 1/1000 dilution + Mouse kidney cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 14 kDa
      Observed band size : 18 kDa (why is the actual band size different from the predicted?)
    • ab109211, at 1/50 dilution staining Sca1 / Ly6A/E in paraffin-embedded Mouse kidney tissue by Immunohistochemistry.
    • Immunohistochemical analysis of murine bone marrow tissue, following total body irradiation, staining Sca1 / Ly6A/E with ab109211. Sections were blocked with blocking reagent before staining with primary antibody and a peroxidase conjugated anti-rabbit IgG. Staining was detected with AEC substrate.

    References

    This product has been referenced in:
    • Natale BV  et al. Sca-1 identifies a trophoblast population with multipotent potential in the mid-gestation mouse placenta. Sci Rep 7:5575 (2017). Read more (PubMed: 28717241) »
    • Okamoto M  et al. Stem Cell Antigen 1-Positive Mesenchymal Cells Are the Origin of Follicular Cells during Thyroid Regeneration. PLoS One 8:e80801 (2013). IHC-P ; Mouse . Read more (PubMed: 24278321) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    Thank you for contacting us. I have heard back from the lab: For WB, denaturing and reducing conditions were used. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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