The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: Use at an assay dependent dilution.
IHC-P: Use at a concentration of 5 µg/ml.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 108 kDa.
N.B. on SDS PAGE gels, Scaffold attachment factor B2 runs as an approximately 130 kDa protein. This aberrant migration is due to a large number of charged amino acid residues.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionBinds to scaffold/matrix attachment region (S/MAR) DNA. Can function as an estrogen receptor corepressor and can also inhibit cell proliferation.
Tissue specificityExpressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines.