Anti-Scaffold attachment factor B2 antibody (ab104220)

Overview

  • Product name
    Anti-Scaffold attachment factor B2 antibody
    See all Scaffold attachment factor B2 primary antibodies
  • Description
    Rabbit polyclonal to Scaffold attachment factor B2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 351-502 of Human Scaffold attachment factor B2 (AAH25279).

  • Positive control
    • 293T, A431 and Jurkat cell lines. HBL435 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab104220 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 107 kDa.
IHC-P 1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer ( PH8.0).
ICC/IF 1/100 - 1/200.

Target

  • Function
    Binds to scaffold/matrix attachment region (S/MAR) DNA. Can function as an estrogen receptor corepressor and can also inhibit cell proliferation.
  • Tissue specificity
    Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines.
  • Sequence similarities
    Contains 1 RRM (RNA recognition motif) domain.
    Contains 1 SAP domain.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZP779C1727 antibody
    • HAP antibody
    • HET antibody
    • hsp27 ere tata binding antibody
    • KIAA0138 antibody
    • SAF B antibody
    • SAF B2 antibody
    • SAF-B2 antibody
    • SAFB antibody
    • SAFB1 antibody
    • SAFB2 antibody
    • SAFB2_HUMAN antibody
    • Scaffold attachment factor B2 antibody
    see all

Images

  • Anti-Scaffold attachment factor B2 antibody (ab104220) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 107 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed A431, using ab104220 at 1/200 dilution. Lower picture costained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using ab104220 at 1/100 dilution.

References

ab104220 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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