Recombinant Anti-SCAI antibody [EPR4128] (ab124688)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4128] to SCAI
- Suitable for: IHC-P, Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-SCAI antibody [EPR4128]
See all SCAI primary antibodies -
Description
Rabbit monoclonal [EPR4128] to SCAI -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, Flow Cyt (Intra), ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human thymus, thyroid, and 293T cell lysates; SH-SY5Y cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 5.80 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4128 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124688 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
For unpurified, use 1/5. |
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Flow Cyt (Intra) |
1/160.
For unpurified, use 1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
ICC/IF |
1/400.
For unpurified, use 1/50. |
|
WB |
1/1000 - 1/2000. Detects a band of approximately 66 kDa (predicted molecular weight: 70 kDa).
For unpurified, use 1/200. |
Notes |
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IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For unpurified, use 1/5. |
Flow Cyt (Intra)
1/160. For unpurified, use 1/20. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/400. For unpurified, use 1/50. |
WB
1/1000 - 1/2000. Detects a band of approximately 66 kDa (predicted molecular weight: 70 kDa). For unpurified, use 1/200. |
Target
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Function
Tumor suppressor which functions to suppress MKL1-induced SRF transcriptional activity. May function in the RHOA-DIAPH1 signal transduction pathway and regulate cell migration through transcriptional regulation of ITGB1. -
Sequence similarities
Belongs to the SCAI family. -
Cellular localization
Membrane. Nucleus. Cytoplasm. Nuclear localization is required for inhibition of MKL1. - Information by UniProt
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Database links
- Entrez Gene: 286205 Human
- Entrez Gene: 320271 Mouse
- Entrez Gene: 690538 Rat
- SwissProt: Q8N9R8 Human
- SwissProt: Q8C8N2 Mouse
- Unigene: 59504 Human
- Unigene: 204836 Mouse
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Alternative names
- C9orf126 antibody
- FLJ11758 antibody
- FLJ36664 antibody
see all
Images
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Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with purified ab124688 at a dilution of 1/400. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.
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All lanes : Anti-SCAI antibody [EPR4128] (ab124688) at 1/1600 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Anti-SCAI antibody [EPR4128] (ab124688) at 1/1600 dilution (purified) + Human thyroid tissue lysate at 20 µg
Secondary
HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunohistochemical staining of paraffin embedded human brain with purified ab124688 at a working dilution of 1 in 50. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
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Overlay histogram showingSH-SY5Y cells fixed in 2% PFA and stained with purified ab124688 at a dilution of 1 in160 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.
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All lanes : Anti-SCAI antibody [EPR4128] (ab124688) at 1/1000 dilution (unpurified)
Lane 1 : Human Thymus lysate
Lane 2 : Human Thyroid lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 70 kDa -
All lanes : Anti-SCAI antibody [EPR4128] (ab124688) at 1/200 dilution (purified)
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 66 kDa why is the actual band size different from the predicted?Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
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Immunohistochemical staining of paraffin embedded human brain with unpurified ab124688 at a working dilution of 1 in 5. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.
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Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with unpurified ab124688 at a dilution of 1/50. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.
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Unpurified ab124688 at 1/100 dilution staining SCAI in SH-SY5Y cells by intracellular flow cytometry (red) compared to a nonspecific negative control antibody (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab124688 has been referenced in 4 publications.
- Zhang Y et al. Exosomal CircGDI2 Suppresses Oral Squamous Cell Carcinoma Progression Through the Regulation of MiR-424-5p/SCAI Axis. Cancer Manag Res 12:7501-7514 (2020). PubMed: 32943917
- Zhao Z et al. Circular RNA Cdr1as Upregulates SCAI to Suppress Cisplatin Resistance in Ovarian Cancer via miR-1270 Suppression. Mol Ther Nucleic Acids 18:24-33 (2019). PubMed: 31479922
- Zou Y et al. miR-34a is downregulated in human osteosarcoma stem-like cells and promotes invasion, tumorigenic ability and self-renewal capacity. Mol Med Rep 15:1631-1637 (2017). ICC/IF ; Human . PubMed: 28260055
- Krawczyk KM et al. Injury induced expression of caveolar proteins in human kidney tubules - role of megakaryoblastic leukemia 1. BMC Nephrol 18:320 (2017). PubMed: 29065889