Overview

  • Product nameAnti-SCAI antibody [EPR4128]
    See all SCAI primary antibodies
  • Description
    Rabbit monoclonal [EPR4128] to SCAI
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acid residues in Human SCAI.

  • Positive control
    • Human thymus, thyroid, and 293T cell lysates; SH-SY5Y cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab124688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

For unpurified, use 1/5.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

ICC/IF 1/400.

For unpurified, use 1/50.

WB 1/1000 - 1/2000. Detects a band of approximately 66 kDa (predicted molecular weight: 70 kDa).

For unpurified, use 1/200.

Flow Cyt 1/160.

For unpurified, use 1/20.





ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionTumor suppressor which functions to suppress MKL1-induced SRF transcriptional activity. May function in the RHOA-DIAPH1 signal transduction pathway and regulate cell migration through transcriptional regulation of ITGB1.
  • Sequence similaritiesBelongs to the SCAI family.
  • Cellular localizationMembrane. Nucleus. Cytoplasm. Nuclear localization is required for inhibition of MKL1.
  • Information by UniProt
  • Database links
  • Alternative names
    • C9orf126 antibody
    • FLJ11758 antibody
    • FLJ36664 antibody
    • MGC120525 antibody
    • MGC120526 antibody
    • MGC120528 antibody
    • NET40 antibody
    • Protein SCAI antibody
    • SCAI antibody
    • SCAI_HUMAN antibody
    • Suppressor of cancer cell invasion antibody
    • Suppressor of cancer cell invasion protein antibody
    see all

Anti-SCAI antibody [EPR4128] images

  • All lanes : Anti-SCAI antibody [EPR4128] (ab124688) at 1/1600 dilution (purified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 70 kDa
    Observed band size : 66 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-SCAI antibody [EPR4128] (ab124688) at 1/1600 dilution (purified) + Human thyroid tissue lysate at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 70 kDa
    Observed band size : 66 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded human brain with purified ab124688 at a working dilution of 1 in 50. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

  • Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with purified ab124688 at a dilution of 1/400. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

  • Overlay histogram showing SH-SY5Y cells fixed in 2% PFA and stained with purified ab124688 at a dilution of 1 in 160 (pink line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 150. Rabbit monoclonal IgG was used as an isotype control.

  • All lanes : Anti-SCAI antibody [EPR4128] (ab124688) at 1/1000 dilution (unpurified)

    Lane 1 : Human Thymus lysate
    Lane 2 : Human Thyroid lysate
    Lane 3 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat-anti-rabbit HRP at 1/2000 dilution

    Predicted band size : 70 kDa
  • All lanes : Anti-SCAI antibody [EPR4128] (ab124688) at 1/200 dilution (purified)

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size : 70 kDa
    Observed band size : 66 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded human brain with unpurified ab124688 at a working dilution of 1 in 5. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0.

  • Immunofluorescent staining of MCF7 cells (fixed with 4% PFA) with unpurified ab124688 at a dilution of 1/50. An Alexa Fluor® 488 goat anti-rabbit antibody was used as the secondary at a dilution of 1/200. The panel on the right shows the DAPI counter-staining.

  • Unpurified ab124688 at 1/100 dilution staining SCAI in SH-SY5Y cells by Flow cytometry (red) compared to a nonspecific negative control antibody (green).

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References for Anti-SCAI antibody [EPR4128] (ab124688)

ab124688 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"