Overview

  • Product nameAnti-SCAMP5 antibody
    See all SCAMP5 primary antibodies
  • Description
    Rabbit polyclonal to SCAMP5
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Cow
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Rat SCAMP5 aa 217-233.
    Sequence:

    NQPQTQYSATPNYTYSN


    (Peptide available as ab4971)

  • Positive control
    • Rat brain extract.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab3432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa.Can be blocked with SCAMP5 peptide (ab4971).
ICC/IF Use at an assay dependent concentration. PubMed: 19240033

Target

  • FunctionRequired for the calcium-dependent exocytosis of signal sequence-containing cytokines such as CCL5. Probably acts in cooperation with the SNARE machinery. May play a role in accumulation of expanded polyglutamine (polyQ) protein huntingtin (HTT) in case of endoplasmic reticulum stress by inhibiting the endocytosis pathway.
  • Tissue specificityExpressed both by neuronal and nonneuronal tissues. Expressed in brain, stomach, thyroid, spinal cord, lymph node, trachea, adrenal gland, bone marrow and in the different parts of brain. In thyroid tissues, it is expressed by the follicular epithelial cells. In the adrenal gland tissues it is detected in the zona fasciculate of the cortex region (at protein level).
  • Sequence similaritiesBelongs to the SCAMP family. SCAMP5 subfamily.
  • Cellular localizationCell membrane. Golgi apparatus membrane. Golgi apparatus > trans-Golgi network membrane. Recycling endosome membrane. Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Mainly localizes in Golgi apparatus membrane. Upon calcium-triggered exocytosis, it translocates to the cell membrane. Highly enriched in synaptic vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • AI426171 antibody
    • AW558254 antibody
    • DKFZp686L1799 antibody
    • hSCAMP5 antibody
    • MGC24969 antibody
    • Sc5 antibody
    • SCAM5_HUMAN antibody
    • Scamp5 antibody
    • Secretory carrier associated membrane protein 5 antibody
    • Secretory carrier membrane protein 5 antibody
    • Secretory carrier-associated membrane protein 5 antibody
    see all

Anti-SCAMP5 antibody images

  • ab3432 staining SCAMP5 in striatal neurons of R6/2 mouse by immunocytochemistry/ immunofluorescence. Cells were cultured from newborn R6/2 mice at day 2 and maintained in vitro for 3 days. Thereafter, cells were incubated with dimethyl sulfoxide (DMSO) (cells in left image) or 0.5 μm thapsigargin (cells in right image) for 24 h. Cells were grown on a coverslip coated with poly-l-lysine, fixed with 4% paraformaldehyde, and permeabilized with 0.01% Triton X-100. After blocking with 3% bovine serum albumin, the samples were incubated with primary antibody for 1 hour and fluorphore conjugated secondary antibodies. Red color stainig show positive staining with ab3432, while green color indiacates satining with antibody against ubiquitin.

  • Anti-SCAMP5 antibody (ab3432) + Rat brain extract

    Observed band size : 25 kDa (why is the actual band size different from the predicted?)

References for Anti-SCAMP5 antibody (ab3432)

This product has been referenced in:
  • Noh JY  et al. SCAMP5 Links Endoplasmic Reticulum Stress to the Accumulation of Expanded Polyglutamine Protein Aggregates via Endocytosis Inhibition. J Biol Chem 284:11318-25 (2009). ICC/IF . Read more (PubMed: 19240033) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"