1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway.
Belongs to the WD repeat SCAP family. Contains 1 SSD (sterol-sensing) domain. Contains 7 WD repeats.
Cholesterol bound to SSC domain of SCAP or oxysterol bound to INSIG1/2 leads to masking of an ER export signal on SCAP possibly by moving the signal further away from the ER membrane.
Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
Flow cytometry analysis of K562 cells labeling SCAP with ab170674 at 1/100 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human stomach tissue labeling SCAP with ab170674 at 1/10 dilution, followed by a peroxidase conjugated secondary antibody and DAB staining.
Western blot - Anti-SCAP antibody - N-terminal (ab170674)
Anti-SCAP antibody - N-terminal (ab170674) at 1/100 dilution + K562 cell lysate at 35 µg