• Product name
    Anti-SCAP antibody - N-terminal
    See all SCAP primary antibodies
  • Description
    Rabbit polyclonal to SCAP - N-terminal
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SCAP aa 53-83 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q12770

  • Positive control
    • K562 cell lysate; Human stomach tissue; K562 cells.



Our Abpromise guarantee covers the use of ab170674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 140 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.



  • Function
    Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP/SREBF complex from the ER upon low cholesterol. Formation of a ternary complex with INSIG at high sterol concentrations leads to masking of an ER-export signal in SCAP and retention of the complex in the ER. Low sterol concentrations trigger release of INSIG, a conformational change in the SSC domain of SCAP, unmasking of the ER export signal, recruitment into COPII-coated vesicles, transport to the Golgi complex, proteolytic cleavage of SREBF in the Golgi, release of the transcription factor fragment of SREBF from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway.
  • Sequence similarities
    Belongs to the WD repeat SCAP family.
    Contains 1 SSD (sterol-sensing) domain.
    Contains 7 WD repeats.
  • Domain
    Cholesterol bound to SSC domain of SCAP or oxysterol bound to INSIG1/2 leads to masking of an ER export signal on SCAP possibly by moving the signal further away from the ER membrane.
  • Cellular localization
    Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA0199 antibody
    • SCAP antibody
    • SCAP_HUMAN antibody
    • SREBF chaperone antibody
    • SREBF chaperone protein antibody
    • SREBP cleavage activating protein antibody
    • SREBP cleavage-activating protein antibody
    • Sterol regulatory element binding protein cleavage-activating protein antibody
    • Sterol regulatory element-binding protein cleavage-activating protein antibody
    see all


  • Flow cytometry analysis of K562 cells labeling SCAP with ab170674 at 1/100 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human stomach tissue labeling SCAP with ab170674 at 1/10 dilution, followed by a peroxidase conjugated secondary antibody and DAB staining.

  • Anti-SCAP antibody - N-terminal (ab170674) at 1/100 dilution + K562 cell lysate at 35 µg

    Predicted band size: 140 kDa


ab170674 has not yet been referenced specifically in any publications.

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