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Goat polyclonal to SCARF1
This antibody is expected to recognise all 5 isoforms of SCARF1.
, corresponding to amino acids 20 - 31 of Human SCARF1.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration information loading...
Immunogen affinity purified
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution. PubMed: 20606164
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 85-90 kDa (predicted molecular weight: 87 kDa).
Mediates the binding and degradation of acetylated low density lipoprotein (Ac-LDL). Mediates heterophilic interactions, suggesting a function as adhesion protein. Plays a role in the regulation of neurite-like outgrowth.
Contains 6 EGF-like domains.
Information by UniProt
Acetyl LDL receptor antibody
Endothelial cells scavenger receptor antibody
Western blot - Anti-SCARF1 antibody (ab23933)
All lanes :
Anti-SCARF1 antibody (ab23933) at 0.3 µg/ml
Lane 1 :
(A) Human Heart lysate
Lane 2 :
(B) Human Heart lysate with immunizing peptide
Lysates/proteins at 35 µg per lane.
Predicted band size:
Observed band size:
85-90 kDa (
why is the actual band size different from the predicted?
Additional bands at:
28 kDa (possible non-specific binding)
Primary incubation was 1 hour. Detected by chemiluminescence.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"