Recombinant
RabMAb

Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629)

Overview

  • Product name
    Anti-Scavenging Receptor SR-BI antibody [EP1556Y]
    See all Scavenging Receptor SR-BI primary antibodies
  • Description
    Rabbit monoclonal [EP1556Y] to Scavenging Receptor SR-BI
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Rat, Sheep, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Scavenging Receptor SR-BI aa 50-150 (N terminal). The exact sequence is proprietary.
    Database link: Q8WTV0

  • Positive control
    • WB: Mouse liver tissue lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab52629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 80 kDa.
IHC-P 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Receptor for hepatitis C virus glycoprotein E2. Binding between SCARB1 and E2 was found to be independent of the genotype of the viral isolate. Plays an important role in the uptake of HDL cholesteryl ester.
    • Tissue specificity
      Widely expressed.
    • Sequence similarities
      Belongs to the CD36 family.
    • Post-translational
      modifications
      N-glycosylated.
    • Cellular localization
      Cell membrane. Membrane > caveola. Predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane, called caveolae.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD36 and LIMPII analogous 1 antibody
      • CD36 antibody
      • CD36 Antigen like 1 antibody
      • CD36 antigen-like 1 antibody
      • CD36L1 antibody
      • CLA 1 antibody
      • CLA-1 antibody
      • CLA1 antibody
      • Collagen type I receptor antibody
      • HDLQTL6 antibody
      • MGC138242 antibody
      • SCARB1 antibody
      • Scavebger Receptor Class B Member 1 antibody
      • Scavenger receptor class B member 1 antibody
      • Scavenger Receptor Class B Type 1 antibody
      • SCRB1_HUMAN antibody
      • SR BI antibody
      • SR-BI antibody
      • SRB1 antibody
      • SRBI antibody
      • Thrombospondin receptor like 1 antibody
      • thrombospondin receptor-like 1 antibody
      see all

    Images

    • All lanes : Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/1000 dilution (purified)

      Lane 1 : Mouse liver lysate
      Lane 2 : Rat liver lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Observed band size : 80 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/1000 dilution (purified)

      Lane 1 : Human fetal liver lysate
      Lane 2 : HepG2 lysate
      Lane 3 : PC-3 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Anti-rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Observed band size : 80 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST
      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemical staining of paraffin embedded human liver with purified ab52629 at a working dilution of 1/500. The secondary antibody used is ab97051, a goat anti-rabbit IgG (H&L) at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
    • Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629) at 1/2000 dilution (unpurified) + Mouse liver lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/2000 dilution

      Observed band size : 80 kDa (why is the actual band size different from the predicted?)

    • Developed using the ECL technique

      Performed under reducing conditions.

      THP1 cells were incubated at 37ºC for 40h with vehicle control (0 µM) and different concentrations of sodium salicylate (ab120746). Decreased expression of scavenging receptor SR-BI in THP1 cells correlates with an increase in sodium salicylate concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with unpurified ab52629 at 1/2000 dilution and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

       

    References

    This product has been referenced in:
    • Johnson R  et al. SR-B1-targeted nanodelivery of anti-cancer agents: a promising new approach to treat triple-negative breast cancer. Breast Cancer (Dove Med Press) 9:383-392 (2017). Flow Cyt . Read more (PubMed: 28670138) »
    • Liu MX  et al. Dicer1/miR-29/HMGCR axis contributes to hepatic free cholesterol accumulation in mouse non-alcoholic steatohepatitis. Acta Pharmacol Sin 38:660-671 (2017). Read more (PubMed: 28112179) »

    See all 17 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Spleen)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    40 µg
    Specification
    Spleen
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 21 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293T)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    HEK293T
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 14 2017

    EThank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody, particularly as the previous one you were using succesfully is no longer available.

    I would like to r...

    Read More

    Thank you for contacting us this morning.

    I can now confirm that the concentration of Anti-Scavenging Receptor SR-BI antibody [EP1556Y] (ab52629), lot number GR52658-2 is0.223 mg/mL.

    I hope this information has been of help. If you ...

    Read More

    Thank you for contacting us. This product has an 85% homolgy between the immunogen and rat protein. The sequence is I have plaved a link to blastp for rat below: http://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE_TYPE=BlastSearch&PROG_DEF=blastn...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Flow Cytometry
    Sample
    Mouse Cell (macrophage cell line RAW267.4)
    Permeabilization
    No
    Gating Strategy
    macrophages
    Specification
    macrophage cell line RAW267.4
    Preparation
    Cell harvesting/tissue preparation method: by cell scraper
    Sample buffer: 1x PBS
    Fixation
    Formaldehyde
    Username

    Mr. Pijus Barman

    Verified customer

    Submitted May 17 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up