The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
Use at 2 µg/mg of lysate.
Probable regulator of sister chromatid cohesion in mitosis which may stabilize cohesin complex association with chromatin. May couple sister chromatid cohesion during mitosis to DNA replication. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.
Highest level in colon. Low levels in lung, ovary, breast and kidney. Reduced level in renal tumor tissue. Isoform 2 is expressed in kidney.
Belongs to the PDS5 family. Contains 1 HEAT repeat.
Cell cycle-regulated with highest level in G2 phase.
Nucleus. Associated with chromatin through most of the cell cycle. Dissociates from chromatin in late prophase, reassociates during late telophase.
Cell proliferation-inducing gene 54 protein antibody
PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae) antibody
Sister chromatid cohesion protein 112 antibody
Sister chromatid cohesion protein PDS5 homolog A antibody
Western blot - SCC112 antibody (ab17960)
Predicted band size : 150 kDa
Western blot and IP using ab17960 at 0.1µg/ml for Western blot and at 2µg/ml for IP.
Lane 1: WB; 5 µg of NE
Lane 2: WB; 20 µg of NE
Lane 3: IP (with ab17960) followed by WB; 1 mg of NE;
Lane 4: IP (with a different antibody) followed by WB; 1 mg of NE
5 to 20µg of nuclear extract (NE) from HeLa cell lysate were used for WB. 1 mg of nuclear extract (NE) from HeLa cell lysate were used for IP.
Detection: Chemiluminescence with an exposure time of less than 5 minutes.