Overview

  • Product name
  • Description
    Rabbit polyclonal to SCC112
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    The immunogen is within the region between residues 1150 and 1200 of human SCC-112.

Properties

Applications

Our Abpromise guarantee covers the use of ab17960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
IP Use at 2 µg/mg of lysate.

Target

  • Function
    Probable regulator of sister chromatid cohesion in mitosis which may stabilize cohesin complex association with chromatin. May couple sister chromatid cohesion during mitosis to DNA replication. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.
  • Tissue specificity
    Highest level in colon. Low levels in lung, ovary, breast and kidney. Reduced level in renal tumor tissue. Isoform 2 is expressed in kidney.
  • Sequence similarities
    Belongs to the PDS5 family.
    Contains 1 HEAT repeat.
  • Developmental stage
    Cell cycle-regulated with highest level in G2 phase.
  • Cellular localization
    Nucleus. Associated with chromatin through most of the cell cycle. Dissociates from chromatin in late prophase, reassociates during late telophase.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation inducing protein 54 antibody
    • Cell proliferation-inducing gene 54 protein antibody
    • KIAA0648 antibody
    • PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae) antibody
    • PDS5A antibody
    • PDS5A_HUMAN antibody
    • PIG54 antibody
    • SCC-112 antibody
    • SCC112 antibody
    • Sister chromatid cohesion protein 112 antibody
    • Sister chromatid cohesion protein PDS5 homolog A antibody
    see all

Images



  • Predicted band size : 150 kDa


    Western blot and IP using ab17960 at 0.1µg/ml for Western blot and at 2µg/ml for IP.

    Lane 1: WB; 5 µg of NE
    Lane 2: WB; 20 µg of NE
    Lane 3: IP (with ab17960) followed by WB; 1 mg of NE;
    Lane 4: IP (with a different antibody) followed by WB; 1 mg of NE

    5 to 20µg of nuclear extract (NE) from HeLa cell lysate were used for WB. 1 mg of nuclear extract (NE) from HeLa cell lysate were used for IP.
    Detection: Chemiluminescence with an exposure time of less than 5 minutes.

References

This product has been referenced in:
  • Stoepker C  et al. Defects in the Fanconi Anemia Pathway and Chromatid Cohesion in Head and Neck Cancer. Cancer Res 75:3543-53 (2015). Read more (PubMed: 26122845) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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