Overview

  • Product name
  • Description
    Rabbit polyclonal to SCC112
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human SCC112 aa 1250-1297 (C terminal).
    Database link: 23244
    (Peptide available as ab40229)

Properties

Applications

Our Abpromise guarantee covers the use of ab17961 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/25000. Detects a band of approximately 145 kDa (predicted molecular weight: 150 kDa).
IP Use a concentration of 1 - 4 µg/ml.

Target

  • Function
    Probable regulator of sister chromatid cohesion in mitosis which may stabilize cohesin complex association with chromatin. May couple sister chromatid cohesion during mitosis to DNA replication. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.
  • Tissue specificity
    Highest level in colon. Low levels in lung, ovary, breast and kidney. Reduced level in renal tumor tissue. Isoform 2 is expressed in kidney.
  • Sequence similarities
    Belongs to the PDS5 family.
    Contains 1 HEAT repeat.
  • Developmental stage
    Cell cycle-regulated with highest level in G2 phase.
  • Cellular localization
    Nucleus. Associated with chromatin through most of the cell cycle. Dissociates from chromatin in late prophase, reassociates during late telophase.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell proliferation inducing protein 54 antibody
    • Cell proliferation-inducing gene 54 protein antibody
    • KIAA0648 antibody
    • PDS5, regulator of cohesion maintenance, homolog A (S. cerevisiae) antibody
    • PDS5A antibody
    • PDS5A_HUMAN antibody
    • PIG54 antibody
    • SCC-112 antibody
    • SCC112 antibody
    • Sister chromatid cohesion protein 112 antibody
    • Sister chromatid cohesion protein PDS5 homolog A antibody
    see all

Images



  • Predicted band size : 150 kDa


    Detection of Human SCC-112 by Western Blot and Immunoprecipitation.
    Lane 1: WB; 5 µg of NE
    Lane 2: WB; 20 µg of NE
    Lane 3: IP (with a different antibody) followed by WB; 1 mg of NE
    Lane 4: IP (with ab17961) followed by WB; 1 mg of NE 5 to 20µg of nuclear extract (NE) from HeLa cell lysate were used for WB. 1 mg of nuclear extract (NE) from HeLa cell lysate were used for IP.
    Detection: Chemiluminescence with an exposure time of less than 5 minutes.

    Ab17961 was used at a dilution of 1/10,000 for the western blot and 2 µg/mg of extract for immunoprecipitation.

References

ab17961 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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