Overview

  • Product nameAnti-Scc4 antibody
    See all Scc4 primary antibodies
  • Description
    Rabbit polyclonal to Scc4
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 564-613 (MHQNFSQQLL QDHIEACSLP EHNLITWTDG PPPVQFQAQN GPNTSLASLL) of Human Scc4 (NP_056144)

  • Positive control
    • MCF7 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab83261 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 69 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

Target

  • RelevanceScc4 (Mau2) is the metazoan homologue of Scc4 in S. cerevisiae. In yeast, Scc4 binds to Scc2 to form an essential complex that loads cohesion onto chromosomes. Similarly, the Scc4 / Mau2 protein in metazoans plays a significant role in sister chromatid separation and in loading of cohesin onto chromatin. The Scc2-Scc4 complex is tethered to pre-replication complexes through the Cdc7-Drf1 protein kinase DDK.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • K0892 antibody
    • KIAA0892 antibody
    • Mau 2 antibody
    • Mau 2L antibody
    • mau-2 antibody
    • Mau2 antibody
    • MAU2L antibody
    • Scc 4 antibody
    • SCC4 antibody
    • sister chromatid cohesion 4 antibody
    see all

Anti-Scc4 antibody images

  • Anti-Scc4 antibody (ab83261) at 1 µg/ml (5% skim milk / PBS buffer) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 69 kDa
    Observed band size : 64 kDa (why is the actual band size different from the predicted?)
    Additional bands at : >90 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Scc4 antibody (ab83261)

ab83261 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Permeabilization Yes - 0.5% TritonX100 in PBS
Specification HeLa
Fixative Paraformaldehyde
Username

Dr. Kirk McManus

Verified customer

Submitted Jun 18 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"