Overview

  • Product nameAnti-SCD1 antibody
    See all SCD1 primary antibodies
  • Description
    Rabbit polyclonal to SCD1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Goat, Guinea pig, Cat, Dog
  • Immunogen

    A region within a synthetic peptide: HVGWLLVRKH PAVKEKGSTL DLSDLEAEKL VMFQRRYYKP GLLMMCFILP, corresponding to internal sequence amino acids 181-230 of Human SCD

  • Positive control
    • Fetal liver lysate Human liver tissue slide

Applications

Our Abpromise guarantee covers the use of ab23331 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.

Target

  • FunctionTerminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA.
  • Sequence similaritiesBelongs to the fatty acid desaturase family.
  • DomainThe histidine box domains may contain the active site and/or be involved in metal ion binding.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ACOD_HUMAN antibody
    • Acyl-CoA desaturase antibody
    • Delta(9)-desaturase antibody
    • Delta-9 desaturase antibody
    • Delta-9-Desaturase antibody
    • FADS5 antibody
    • Fatty acid desaturase antibody
    • PRO0998 antibody
    • SCD 1 antibody
    • SCD antibody
    • SCD1 antibody
    • Stearoyl-CoA desaturase antibody
    see all

Anti-SCD1 antibody images

  • Anti-SCD1 antibody (ab23331) at 1 µg/ml + Fetal liver lysate

    Secondary
    HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000

    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling SCD1 with ab23331 at a concentration of 4-8µg/ml. Cells with positive staining are marked by arrows. Magnification: X400.

References for Anti-SCD1 antibody (ab23331)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Non-reduced Denaturing (PAGE 12%)
Sample Cow Cell lysate - whole cell (bovine fetal liver lysate)
Specification bovine fetal liver lysate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jun 09 2014

Thank you for contacting us and for your interest in our products.

As far as we are aware, ab23331 has never been tested in ELISA so we do not know whether or not it is suitable for this application. All tested applications are specified on ...

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Thank you for contacting us.

According to SwissProt, the predicted MW of the SCD human protein is 41.5 kDa.

http://www.uniprot.org/uniprot/O00767

We made a note on the datasheet to point out that the band observed at 37 k...

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DISCOUNT CODE: ***
Expiration date: September 11th, 2012

I am very pleased to hear you would like to accept our offer and test ab23331 in rat. This code will give you: 1 free primary antibody before the expiration date. To redeem this of...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (liver microsomes)
Loading amount 10 µg
Specification liver microsomes
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1µg/mL · Temperature: 25°C
Username

Dr. Colin Shearn

Verified customer

Submitted Jan 27 2012

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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The peptide used was derived from that region - residues 184-238 of Human SCD. The antibody has not been tested against the SCD2 or SCD5 isoform. Please contact us again if you have any additional questions.

I was informed that the following method was used with ab23331 immunostaining of human liver sections: 1. Deparaffinize sections in 3 changes of xylene, 5 minutes each 2. Hydrate in 2 changes of 100% ethanol for 3 minutes each, 95% and 80% ethan...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"